摘要
目的分析基因芯片法检测结核分枝杆菌（MTB）耐药性的灵敏度、特异度及符合率,为临床提供方便、准确、快速的MTB耐药性的检测方法。方法以DNA测序法为金标准,采用基因芯片法与比例法药敏试验同时对2014年8~12月收治的250例结核病患者的痰液标本分离MTB菌株进行耐药性检测,比较两种方法检测MTB对利福平和异烟肼耐药性的效能。结果基因芯片法与比例法药敏试验检测MTB对利福平的耐药率分别为3.0%、3.5%,对异烟肼的耐药率分别为6.7%、8.2%。以DNA测序法为金标准,基因芯片法检测MTB对利福平与异烟肼耐药性的灵敏度、特异度及符合率均高于比例法药敏试验,且检测时间短于比例法药敏试验,差异均有统计学意义（P〈0.05）。结论运用基因芯片法检测MTB对利福平和异烟肼的耐药性,具有较高的灵敏度、特异度与符合率,可以代替比例法药敏试验成为检测MTB耐药性的有效方法。
Objective To analyse the sensitivity,specificity and coincidence rate of genechip method in the detection of resistance to antibacterial agents in Mycobacterium tuberculosis（MTB）,in order to provide a convenient,accurate and rapid method for detecting antibacterial resistance in MTB.Methods The DNA sequencing was taken as gold standard,and antibacterial resistance of the strains of MTB isolated from sputum specimens of 250 cases of patients with tuberculosis from August to December 2014 were detected by using the genechip method and proportion method for susceptibility testing at the same time.Efficacies of the two methods in detecting MTB resistance to rifarnpin and isoniazid were compared.Results The MTB resistance rate to rifarnpin detected by using the genechip method and proportion method for susceptibility testing was 3.0% and 3.5% respectively;that to isoniazid was 6.7% and 8.2% respectively.For detecting MTB resistance to rifarnpin and isoniazid,the DNA sequencing was taken as gold standard,the sensitivity,specificity and coincidence rate of genechip method was higher than those of proportion method for susceptibility testing,and the test time of genechip method was shorter than that of proportion method for susceptibility testing,there were statistically significant differences（P〈0.05）.Conclusion Using the genechip method to detecting MTB resistance to rifampin and isoniazid has high sensitivity,specificity and coincidence rate,which could replace the proportion method for susceptibility testing and become an effective method.
出处
《国际检验医学杂志》
CAS
2015年第23期3421-3422,3425,共3页
International Journal of Laboratory Medicine
基金
湖北省宜昌市卫生科技项目(No.A15301-38)
关键词
基因芯片法
比例法药敏试验
DNA测序法
灵敏度
特异度
符合率
genechip method
proportion method for susceptibility testing
DNA sequencing
sensitivity
specificity
coincidence rate
