摘要
目的：利用降植烷构建一种模拟人类风湿性关节炎发病过程的小鼠模型。方法：6-8周雌性BALB/c小鼠30只,随机分为模型组和对照组,每组15只。模型组于0周、9周、18周时经腹腔注射0.5 ml降植烷;对照组注射0.5 ml生理盐水。观察各组小鼠后足足掌厚度变化,并对关节炎的肿胀程度进行评分;H＆E染色评估滑膜增生和炎性细胞浸润程度;流式细胞术方法检测小鼠脾脏中巨噬细胞、树突状细胞、中性粒细胞、T细胞和B细胞的亚群变化。结果：21周时,模型组有11只小鼠出现足爪红肿,足掌平均厚度为（2.90±0.51）mm,与对照组（1.29±0.47）mm比较,差异有统计学意义（P〈0.05）;关节炎评分为9.55±2.80。H＆E染色结果显示模型组胫骨远端骨小梁吸收破坏,累及骨皮质,关节软骨可见坏死脱落、纤维化,呈现出明显的类风湿关节炎病理改变。流式细胞术分析结果显示,造模21周时,脾脏细胞中CD11b、CD11c和GR1表达明显增加,提示巨噬细胞、树突状细胞和中性粒细胞亚群数量增加;同时,CD4、CD8、CD154的分子表达也明显上调,与对照组比较,差异有统计学意义（P〈0.05）。结论：经腹腔注射降植烷能够成功诱导小鼠关节炎,并产生与RA患者类似的组织病理和免疫学改变。降植烷诱导的小鼠关节炎模型,能够较为准确地模拟临床类风湿关节炎的发病过程。
Objective： To establish a pristine-induced rheumatoid arthritis model in mice,and to evaluate its histological and immunological distinction. Methods： Thirty female BALB/c mice,6-8 weeks old,were randomly divided into 2 groups,a control group and pristine group. The mice in pristine group were injected intraperitoneally with 0. 5 ml pristine three times at 0,9,and 18 weeks,while mice in the control group receiving saline at the same time. Arthritis score and paw thickness were measured and histopathological assessment of joint sections was performed. The expression of phagocytes,dendritic,neutrophils,T and B cells markers in spleen were determined by flow cytometry. Results： In model-marking group,11 mice were presented with macroscopic evidence of arthritis such as erythema or swelling. The paw thickness in pristine-induced mice was significant higher than that in the control groups[（ 2. 90 ±0. 51）mm vs（ 1. 29±0. 47 mm）,P〈0. 05]. In addition,arthritis score in pristine-induced mice was 9. 55±2. 80 at 21 weeks after first injection with 0. 5 ml pristine. HE staining revealed a significant increase of synovial inflammation,cartilage and bone destruction after stimulated with pristine. Meanwhile,the expression levels of CD11 b,CD11c,GR1,CD4,CD8 and CD154 were obviously increased in modelmarking group when compared with that in control group. Conclusion： The pristine-induced model presents the similar histological and immunological distinctions with human rheumatism arthritis,which can mimic the pathogenesis of rheumatism arthritis.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2015年第11期1498-1500,1504,共4页
Chinese Journal of Immunology
基金
国家自然科学基金(No.81373236)
江苏省骨科临床医学研究中心(BL2012004)资助
