摘要
目的探讨特应性皮炎患儿外周血淋巴细胞亚群及相关因子与中医证候的内在相关性,为中医微观辨证提供依据。方法采用ELISA和流式细胞仪分别检测不同证型特应性皮炎患者和正常对照组细胞因子的值及细胞亚群比例。结果AD患者IL-17的量及Th17细胞比例在湿热蕴肤组、IFN-γ的量及Th1比例在血虚风燥组、IL-4的量及Th2比例在湿热蕴肤组及IL-10的量在脾虚湿蕴组均高于其他三组,具有统计学差异。结论在辨证论治过程可以从IL-17、IL-10、IL-4、IFN-γ水平的高低及Th17/Treg、Th1/Th2平衡的偏移方向初步判断患者的病期,大致了解患者的中医分型,有利于中医辨证分型的规范化,有利于中医临床的选方用药的合理性。
Objective To investigate correlations among TCM syndrome, peripheral blood iymphocyte subsets and related factors of atopic dermatitis in Children. Methods Measure the level of eytokine and cell subpopulation of normal controls and AD patients using ELISA and flow cytometry. Results The amount of IL17, IIA and the ratio of Th2 and Th17 cells of AD patients with shireynnfu syndrome were higher than the other groups; The amount of IFN - γ and the ratio of Thl cells of AD patients with xuexufengzao syndrome were higher than the other groups; The amount of IL10 of AD patients with Pixushiyun syndrome were higher than the other groups. The differences among them were statistically significant. Conclusion The TCM syndrome differentia- tion of AD can be learnt based on the level of IL- 17,IL- 10,IL-4,IFN -γ and the balance shift direction of Th17/Treg and Thl/Th2 ,this can be of help to the TCM syndrome differeciation standardization and rational use of medicines of AD.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2015年第11期2801-2804,共4页
Lishizhen Medicine and Materia Medica Research
基金
国家自然科学基金(No.81360599)
广西自然科学基金面上项目(No.2013GXNSFAA019148)
广西壮族自治区卫生厅课题(No.GZZY13-17)
