摘要
目的对葎草中主要黄酮类成分进行提取分离,并对其结构进行鉴定;探讨葎草中黄酮类单体成分对小鼠肺水清除率(AFC)的影响。方法葎草乙醇提取后,除色素,柱分离得单体成分,利用光谱法对其结构进行鉴定;通过气管给药后,酶标仪测定肺内吸出液的小牛血清白蛋白浓度的方法,计算木犀草苷(LGL)和大波斯菊苷(AGL)影响下的小鼠在体AFC。结果葎草中的主要成分为木犀草苷和大波斯菊苷;二者均能促进AFC。结论葎草中的主要成分均可降低肺内的积水。
Aims To extract,separate and identify the flavonoid constituents in Humulus Scandens and to explore the relationship of monomers and alveolar fluid clearance( AFC) in mice in vivo. Methods Humulus scandens were extracted with alcohol and then isolated by the technology of Column and the structures were identified by spectrometry. In vivo AFC was measured using bovine serum albumin protein assays affected by luteolin-7-O-β-D-glucoside( LGL) and cosmsiin( AGL). Results The main constituents of flavanones in Humulus scandens were LGL and AGL. Both of them could improve the AFC. Conclusion The AFCs of LGL and AGL,compared to the blank control group,increased which explains the effect of flavonoid constituents on removing edema and promoting water absorption.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2015年第12期1715-1718,共4页
Chinese Pharmacological Bulletin
基金
辽宁省自然科学基金资助项目(No 2014021038)
关键词
葎草
化学成分
木犀草苷
大波斯菊苷
肺水清除率
肺水肿
Humulus Scandens
chemical constituent
luteolin-7-O-β-D-glucoside
cosmsiin
alveolar fluid clearance(AFC)
pulmonary edema
