摘要
目的:比较组织块法和胶原酶两步灌流法制备大鼠原代肝细胞的产量、活率和活力。方法:通过荧光倒置显微镜观察大鼠原代肝细胞的形态,并且分别通过台盼蓝染色和MTT法测定制备得到的原代肝细胞产量、活率和活力。结果:组织块法和胶原酶两步灌流法制备大鼠原代肝细胞的产量分别为(0.30±0.10)×108个/肝和(0.67±0.11)×108个/肝,活率分别为(65±14)%和(88±16)%,组织块法制备大鼠原代肝细胞的活力比胶原酶两步灌流法更低,且活力下降快。结论:胶原酶两步灌流法制备的大鼠原代肝细胞比组织块法产量更高、活率和活力均更好,更适用于体外药物代谢的研究。
Object： To compare the different of output, living rate and vitality between tissue block method and two steps collagenase perfusion method for preparation ratshepatocytes. Mothod： Observe the shape of rat hepatocytes by fluorescent inverted microscope, and determine the output, living rate and vitality of rats hepatocytes by Trypanblue stainand MTT assay respectively. Results： The output of rats hepatocytes prepared by tissue block method and two steps col lagenase perfusion was （0.30 ±0.10）x 108 per liver and （0.67 ±0.11）x 108 per liver, respectively. The living rate was （65 ±14）% and （88 ±16）%, respectively. The vitality of rats hepatocytes prepared by tissue block method was lower than that by two-step collagenase perfusion method and the vitality down faster. Conclusion＂ The output of rats hepatocytes prepared by two-step collagenase perfusion method was higher than that by tissue block method, as well as living rate and vitality. Two-step collagenase perfusion method is more suitable for the in vitro drug metabolism study.
出处
《药品评价》
CAS
2015年第20期22-25,共4页
Drug Evaluation
关键词
组织块法
胶原酶两步灌流法
大鼠原代肝细胞
产量
活率
活力
Tissue Block Method
Two-step Collagenase Perfusion Method
Output
Rats Hepatocytes
Living rate
Vitality
