摘要
目的探析凝胶法在新生儿溶血病免疫性抗体检测中的临床价值。方法以该院2011年6月—2014年6月所收治85例溶血病疑似新生儿进行研究,分别应用试管法及微柱凝胶法进行溶血病免疫性抗体检测,比较HDN免疫性抗体及HDN免疫性抗体特异性检测阳性率。结果观察组直接抗人球蛋白试验阳性率为81.2%、抗体游离试验阳性率为87.1%、抗体放散试验阳性率为91.8%,符合率为91.8%;对照组对应阳性率分别为64.7%、70.6%、75.3%、75.3%,观察组阳性率显著高于对照组,对比差异有统计学意义(P<0.05)。两种检测方法对免疫性Ig G抗体、Ig G抗A、Ig G抗B、Ig G抗D、Ig G抗E、Ig G抗c E阳性率接近,比较差异无统计学意义(P>0.05)。结论凝胶法检测新生儿溶血病免疫性抗体与试管法相比,所受影响因素更少,敏感性高,操作简单,临床应用价值高。
Objective To explore the clinical value of gel method in detection of hemolytic disease of the newborn immune anti-body in. Methods 85 cases of hemolytic disease of newborn treated in our hospital in June 2011 - June 2014 year in were sus-pected to conduct the research, application of test tube method and detection of hemolytic disease of autoimmune antibodies mi-crocolumn gel test, compared the positive rate of antibody and HDN antibody and specific detection of HDN comparison. Results In observation group, the direct coombs test positive rate was 81.2%, the antibody positive rate was 87.1%, the free test of antibody elution test positive rate was 91.8%, the coincidence rate was 91.8%;the positive rate of control group were 64.7%, 70.6%, corre-sponding to 75.3%, 75.3%, the observation group was significantly higher than those in control group, differences were statistical significance （P〈0.05）. Two methods for the detection of IgG Immunity Antibody and IgG anti A, anti B, anti IgG IgG D, IgG anti E, anti cE positive rate of close to IgG, difference was not statistically significant （P〉0.05）. Conclusion Compared to test tube method, gel method in detection of hemolytic disease of the newborn immune antibody with less factors affecting, high sensitivity, simple operation, high clinical application value.
出处
《中外医疗》
2015年第14期170-171,共2页
China & Foreign Medical Treatment
关键词
凝胶法
试管法
溶血病
免疫性抗体
Gel method
Test tube method
Hemolytic disease
Immune antibody
