摘要
目的：检测慢性粒细胞白血病（CML）病人BMI1 mRNA的表达情况，以探讨其在CML病情进展及预后中的意义。方法：应用定量实时荧光PCR（real-time PCR）技术检测4l例CML病人和10例健康者上述基因的表达。结果：BMI1 mRNA在CML病人的阳性表达率为68.3％，明显高于正常对照组10.0％（P〈0.01），在CML的加速期、急变期的表达水平明显高于慢性期（t =3.421、4.231，P〈0.01）。结论：BMI1基因与CML的病情进展密切相关，是监测CML病情进展及判断预后的有价值的分子标记物。
Objective：To detect the expressions of BMI1 mRNA in chronic myeloid leukemia（CML）and explore their clinical significance.Methods：Using real-time PCR technique,expression of BMI1 mRNA was detected in 41 CML patients and 10 healthy adults.Results： A positive rate of BMI1 in the patients was 68.3%,significantly higher than that of the controls（P〈0.01）. BMI1 expression was much higher in accelerated phase and acute transfomation phase than in chronic phase（t=3.421、4.231, P〈0.01）.Conclusions：The BMI1 gene is closely related to the advancement and prognosis of CML,which is valuable molecular markers to be used for monitoring the disease.
出处
《医学检验与临床》
2015年第5期1-2,8,共3页
Medical Laboratory Science and Clinics
基金
2014年度南通市市级科技计划项目（HS149058）
