摘要
目的:研究有氧运动对2型糖尿病大鼠肾脏VEGF-A、VEGFR2蛋白表达的影响,并对其可能机制进行探讨。方法:通过高糖高脂饲喂5周加低剂量腹腔注射STZ复制2型糖尿病大鼠模型,7周后成模SD大鼠被随机分为糖尿病安静组和糖尿病运动组,另设正常对照组。采用7周有氧游泳运动只对糖尿病运动组进行干预,实验末测定血糖、24 h UA、肾皮质VEGF-A及VEGFR2和p-VEGFR2蛋白的表达及光镜、电镜观察肾小球形态结构的变化。结果:光镜下糖尿病运动组大鼠肾小球增大不明显,毛细血管袢大多数开放,球囊壁渗出、粘连等现象较糖尿病安静组明显改善;基底膜无明显增厚,足突融合等现象较糖尿病安静组均有所减轻;血糖浓度和24 h UA排泄量降低显著(P<0.05或P<0.01);肾皮质VEGF-A、VEGFR2和p-VEGFR2蛋白的表达均较糖尿病安静组显著降低(P<0.05或P<0.01)。结论:运动对2型糖尿病大鼠肾脏有保护作用,其作用机制可能是通过其降低血糖及下调2型糖尿病状态下表达增加的VEGF-A和VEGFR2蛋白水平,而减轻大鼠肾脏损伤。
Objective： To investigate the effect of aerobic exercise training on VEGF-A and VEGFR2 in type 2 diabetic rats and its mechanism. Methods： The model of type 2 diabetic rats was established through SD rats fed high-sugar-fat diet for five weeks together with intraperitoneal infecting after a lowdose of STZ. The28 model rats were randomly divided into diabetic control group（ 14 rats） and diabetic exercise group（ 14 rats）,additionally the normal control group（ 10 rats） were provided after 7 weeks. The diabetic exercise group was intervened of exercise for 7 weeks. We then examined their blood glucose concentrations,the excretion of24 h microalbuminuria（ UA）,and the expressions of VEGF-A,VEGFR2 and p-VEGFR2 in the renal cortex; and the glomerular ultrastructure changes were observed by light microscope and electron microscope. Results： Compared with diabetic control group,1 the exercise group had not an obvious hypertrophy,glomerular wall oozing with the adhesions and part of telangiectasia,nor it had an obvious glomerular basement membrane or foot process fusion,and so on,through light microscope and electron microscope. 2 The concentrations of blood glucose and the excretion of24 h microalbuminuria in diabetic exercise group decreased significantly（ P 〈 0. 05 or P 〈 0. 01）. 3 The expressions of VEGF-A,VEGFR2 and p-VEGFR2 in diabetic exercise group of the renal cortex significantly decreased（ P 〈 0. 05 or P 〈 0. 01）. Conclusion： Exercise could protect renal function and improve renal injury for type 2 diabetes,which may be related to lower blood glucose and hence decrease the protein expression of VEGF-A and VEGFR2 under type 2 diabetes conditions.
出处
《上海体育学院学报》
CSSCI
北大核心
2015年第6期61-67,83,共8页
Journal of Shanghai University of Sport
基金
国家自然科学基金资助项目(31360255
31160217)
井冈山大学自然科学研究项目(JZB1313)
