摘要
目的采用替代对照品法建立镇静安神类药物氯氮?、马来酸咪达唑仑、硝西泮、艾司唑仑、奥沙西泮、劳拉西泮和阿普唑仑的高效液相色谱快速检验方法。方法采用Agilent ZORBAX Eclipse Plus C8色谱柱(4.6 mm×150 mm,5μm),流动相:0.01 mol·L?1磷酸二氢钾溶液(p H 2.5)-(甲醇-乙腈1∶1)(57∶43),流速:1.0 m L·min?1,柱温:30℃。采用相对容量因子和紫外光谱相似度双指标进行定性;采用相对校正因子法进行定量分析。结果在确定的色谱条件下,氯氮?、咪达唑仑、硝西泮、艾司唑仑、奥沙西泮、劳拉西泮和阿普唑仑完全分离;采用紫外光谱相似度和相对容量因子进行定性,结果准确可靠;采用替代对照品法,计算药物的相对校正因子进行含量测定,能有效减少对照品的使用,加快高效液相色谱分析速度。结论该方法快速、简便、可靠,适用于快速检验镇静安神类药物。
OBJECTIVE To establish a rapid HPLC method for sedative drugs（chlordiazepoxide,midazolam,nitrazepam,estazolam, oxazepam, lorazepam and alprazolam） by substitution method of reference substance. METHODS The Agilent ZORBAX Eclipse Plus C8 column（4.6 mm×150 mm, 5 μm） was adopted. The mobile phase was 0.01 mol·L^-1 potassium didydrogen phosphate（p H 2.5）-（methanol-acetonitrile 1 ∶ 1）（57 ∶ 43）. The flow rate was 1.0 m L·min^-1 and the column temperature was 30 ℃. The qualitation was measured by relative retention time and spectral similarity. The relative correction factor for quantification analysis was used for content determination. RESULTS The 7 sedative drugs got complete separation. The qualitation was more accurate by applying the double indicator. The relative correction factor method for quantification which required less reference substances in the on-site screening enabled rapid drug-testing. CONCLUSION This method is rapid, simple and reliable for the rapid determination of sedative drugs.
出处
《中国现代应用药学》
CAS
CSCD
2015年第11期1376-1381,共6页
Chinese Journal of Modern Applied Pharmacy
