摘要
Surface modification may have important influences on the penetration behavior of nanoscale drug delivery system. In the present study, we mainly focused on whether cell targeting or cell penetration could affect penetration abilities of nanostructured lipid carriers（NLC）. Real--time penetration of folate--or cell penetrating peptide（CPP）-modified NLC was evaluated using a multicellular tumor spheroid（MTS） established by stacking culture method as an in vitro testing platform. The results suggested that CPP modification had a better penetration behavior both on penetration depth and intensity compared with folate-modified NLC at the early stage of penetration process.
纳米递药系统的表面修饰作用会对载体的穿透能力产生影响。本研究通过使用叶酸配体和细胞穿膜肽分别修饰的纳米脂质载体,在堆积法建立的肿瘤球模型上实时分析细胞靶向和细胞穿透作用对于纳米脂质载体的肿瘤穿透能力的影响。研究结果表明细胞穿透肽修饰的纳米脂质载体不论是从穿透深度还是强度上,在穿透早期都具备更好的穿透肿瘤球能力。
基金
National key Basic Research Program(Grant No.2013CB932501)
National Natural Science Foundation of China(Grant No.81273454 and 81473156)
Beijing National Science Foundation(Grant No.7132113)
Doctoral Foundation of the Ministry of Education(Grant No.20130001110055)
