摘要
目的：利用Alu-PCR对心肌梗死（MI）患者血浆中游离DNA（cf-DNA）含量进行检测，通过与健康人对比分析，探讨cf-DNA在心梗诊断中的应用及意义。方法收集120例急性心梗（发病后6 h内）以及60例健康志愿者的外周血浆样本，实时荧光定量PCR方法检测MI组、和健康对照组血浆DNA的含量，收集cTnI、MYO、CKMB、LDH等指标，比较分析血浆DNA水平与心梗临床相关性，并作受试者工作特性曲线（ROC）。结果各组血浆DNA浓度MI组和健康对照组血浆DNA含量取对数值后的分别为7.23±1.81（lg copies/ml）和3.79±0.75（lg copies/ml），差异有统计学意义（P〈0.05）。结论血浆cf-DNA具有作为新的心梗诊断标志物的临床应用价值。
Objective To investigate the significance of plasma cell-free DNA （cf-DNA） in diagnosing myocardial infarction（MI）by detecting cf-DNA with Alu-based PCR techniques and comparing the findings with those in healthy subjects. Methods eripheral blood plasma samples were collected from 120 patients with acute myocardial infarction（within 6 h after the onset）and 60 healthy volunteers. Real-time PCR was used to measure the plasma DNA level in samples from the MI group and the healthy control group. Two-site immunnoenzymometric assay was used to measure the levels of cTnI,MYO,CKMB and LDH in the plasma. Receiver operating characteristic curve （ROC） was generated to analyze the correlation between plasma DNA levels and myocardial infarction. Results The logarithmic level of plasma DNA was 7.23 ± 1.81（lg copies/ml）in the MI group vs 3.79 ± 0.75（lg copies/ml）in the healthy control group,and the difference was statistically significant（P〈0.05）. Conclusion Plasma cf-DNA is clinically valuable as a new diagnostic marker of myocardial infarction.
出处
《中华生物医学工程杂志》
CAS
2015年第4期345-348,共4页
Chinese Journal of Biomedical Engineering
基金
上海市松江区科技攻关项目（2012）
