摘要
目的:探讨南蛇藤提取物(Celastrus orbiculatus extract,COE)对人胃癌SGC-7901细胞在裸鼠体内转移的影响及其可能机制。方法:建立裸鼠腹腔转移瘤模型,随机分为对照组,COE低剂量组(10 mg·kg-1)、中剂量组(20 mg·kg-1)、高剂量组(40mg·kg-1),阳性对照组(希罗达)。观察COE对人胃癌SGC-7901细胞在裸鼠体内转移的影响。应用免疫组化法检测腹腔转移瘤组织中E-钙黏蛋白(E-cadherin)、N-钙黏蛋白(N-cadherin)、波形蛋白(Vimentin)的表达。结果:COE各剂量组能够明显抑制腹腔转移瘤的生长、腹膜种植转移和肝肺转移的发生(P<0.05),且呈剂量依赖效应。COE处理后腹腔转移瘤组织中Ecadherin蛋白表达有升高的趋势,而N-cadherin和Vimentin蛋白表达有降低的趋势。其中与对照组相比,COE高剂量组中Ecadherin蛋白表达显著升高(P<0.05),而N-cadherin和Vimentin蛋白表达显著降低(P<0.001)。结论:COE对人胃癌细胞SGC-7901在裸鼠体内的转移具有明显的抑制作用,其机制可能与增高E-cadherin的表达,降低N-cadherin和Vimentin的表达,抑制胃癌EMT的发生有关。
Objective： To investigate the effects and mechanisms of Celastrus orbiculatus extract（ COE） on metastasis of human gastric carcinoma SGC-7901 cells in vivo. Method： SGC-7901 cells were inoculated intraperitoneally in BALB / C nude mice. Two weeks after inoculation,these mice were randomly divided into negative control group（ 1% DMSO）,positive control group（ xeloda）,and low,medium and high dose COE groups（ 10,20 and 40 mg·kg- 1,respectively）. The anti-metastasis effects of COE on nude model mice of gastric cancer were observed and the mechanisms were explored. The expression of EMT markers were evaluated with immunohistochemistry. Results： In COE dose groups the growth of metastases of the abdominal cavity,the occurrence of peritoneal planting metastasis and liver-lung metastasis could be significantly inhitited（ P〈0. 05）,with dose dependent model. Immunohistochemical results suggested that E cadherin protein expression in retroperitoneal metastatic tumor tissue had a tendency to rise after being processed by COE,while N-cadherin and Vimentin protein expression had tendencys to reduce. Compared with the control group,the E-cadherin protein expression of COE high doese group significantly increased（ P〈0. 05）,while the N-cadherin and Vimentin protein expression significantly reduced（ P〈0. 001）. Conclusion： COE is an effective inhibitor of metastatic potential of SGC-7901 cells through suppression of EMT.The mechanism may be related to the increasing expression of E-cadherin,the decreasing expression of N-cadherin and Vimentin,with function to inhibite the pathogenesis of EMT of gastric cancer.
出处
《中医学报》
CAS
2015年第11期1549-1551,共3页
Acta Chinese Medicine
基金
国家自然科学基金(81450051)
国家中医药管理局中医肿瘤学重点学科经费资助项目{国中医药发[2009]30号}
关键词
南蛇藤
胃癌
SGC-7901细胞转移
上皮间质转化
Celastrus orbiculatus
gastric cancer
SGC-7901 cell metastasis
epithelial-mesenchymal transition
