摘要
目的探讨富氢水对重复力竭运动大鼠骨骼肌氧化应激损伤的影响。方法成年雄性SD大鼠100只,随机分为2组:对照组、实验组。重复运动的动物模型构建:大鼠在跑台上进行下坡跑,速度18 m/min,坡度-16度,大强度重复运动(力竭运动)。设立实验组、对照组(实验组运动前30min与运动后即刻口服各10ml体重富氢水;对照组相同时间口服同剂量生理盐水),进行同样的重复力竭运动,在同一时间点分别对对照组、实验组在大强度重复运动(力竭运动)后取股四头肌,同时对实验组大鼠进行治疗量富氢水喂养,考察富氢水对重复运动下大鼠骨骼肌微损伤氧化应激损伤的影响作用,观察对照组、实验组大强度运动(重复运动)下大鼠骨骼肌超微结构变异进行研究。结果补充富氢水后重复运动训练下大鼠骨骼肌超微结构变异研究表明:利用透射电镜对补充富氢水后大鼠进行相同的重复运动(力竭运动)后,该过程中骨骼肌超微结构变化程度看出大鼠股四头肌的微损伤有显著改善,但是仍表现为肌原纤维排列不规则,肌节不完整,Z线变细,肌丝卷曲等。结论大强度运动下骨骼肌超微结构图谱变异与骨骼肌微损伤或者疲劳存在一定的适应性机制,同时对补充富氢水过程中可带来一定的修复机制,富氢水对重复力竭运动大鼠骨骼肌氧化应激损伤具有保护作用。
Objective： To investigate the hydrogen- rich water on skeletal muscle oxidative stress in rats after exhaustive exercise was repeated injury. Methods： 100 adult male SD rats were randomly divided into 2 groups： control group,the experimental group. Construction of an animal model of repetitive motion： bench rats ran downhill running,speed 18 m /min,the slope of- 16 degrees,repeated high- intensity exercise（ exhaustive exercise）. The establishment of the experimental group（ each 10 ml oral immediate weight hydrogen- rich water before and after the experimental group sports and exercise 30min; the same time the control group was treated with the same dose of saline） group,and the same was repeated exhaustive exercise,respectively,at the same time control group,the experimental group were quadriceps strength after repetitive motion（ exhaustive exercise）,while the experimental group were treated rats fed the amount of hydrogen- rich water to investigate hydrogen- rich water damage in rat skeletal muscle micro- oxidation under repetitive motion the influence of stress injury observed in the control group,the experimental group of high- intensity exercise（ repetitive motion） in rat skeletal muscle ultrastructure variation research. Variability of the results of rat skeletal muscle ultrastructure complementary hydrogen- rich water after repeated studies have shown that exercise training： the use of transmission electron microscopy after complementary hydrogen- rich water rats subjected to the same repetitive motion（ exhaustive exercise）,the skeletal muscle in the process over the extent of structural changes in the micro- micro quadriceps injury in rats seen a significant improvement,but still showed myofibrils irregular,incomplete sarcomere,Z thinning,muscle wire curling. Patterns of variation in skeletal muscle ultrastructure and skeletal muscle damage or fatigue micro- adaptive mechanisms exist certain conclusions high intensity exercise,while the complementary process of hydrogen- rich
出处
《泰山医学院学报》
CAS
2015年第4期371-375,共5页
Journal of Taishan Medical College
关键词
富氢水
重复运动
骨骼肌
氧化应激损伤
hydrogen-rich water
repetitive motion
skeletal muscle
oxidative stress
