摘要
目的探讨α-平滑肌肌动蛋白(ɑ-smooth muscle actin,α-SMA)、成纤维细胞活化蛋白(fibroblast-activating protein,FAP)在正常子宫颈、子宫颈上皮内瘤变(cervical intraepithelial neoplasias,CIN)、子宫颈浸润性癌组织的表达,并分析二者表达与子宫颈浸润性癌临床病理指标、浸润转移及预后的关系。方法应用免疫组化Eli Vision两步法检测20例正常子宫颈、20例CIN及98例子宫颈浸润性癌组织中α-SMA和FAP的表达。比较三组中α-SMA和FAP的表达水平,以及二者与子宫颈浸润性癌临床病理特征的相关性。结果α-SMA和FAP在正常子宫颈、CIN中不表达,二者在子宫颈浸润性癌中均表达于肿瘤间质成纤维细胞的胞质内,FAP在肿瘤细胞中有少量表达。α-SMA在子宫颈浸润性癌间质中的阳性率为82.7%(81/98),与临床分期、病理分级和淋巴结转移相关(P<0.05);FAP在子宫颈浸润性癌间质中的阳性率为79.6%(78/98),FAP表达与子宫颈浸润性癌的临床分期、病理分级和淋巴结转移相关(P<0.05);α-SMA和FAP均与患者年龄、肿瘤大小无关(P>0.05)。α-SMA和FAP在子宫颈浸润性癌组织标本中表达在区域上有一致性,在表达强度上呈明显正相关。经直线相关分析α-SMA和FAP呈直线相关(r=0.829,P=0.000)。结论α-SMA和FAP在子宫颈浸润性癌生长、浸润和转移方面发挥一定作用,可作为预测子宫颈浸润性癌预后的可靠指标。
Purpose To detect the expression level of a-smooth muscle actin （α-SMA） and fibroblast-activating protein （FAP） in normal cervix, cervical intraepithelial neoplasms （CIN） , and invasive cervical carcinoma, and to analyze the correlation between α- SMA and FAP expressions and clinicopathological parameters, invasion and metastasis and prognosis. Methods Immunohistochemical EliVision two-steps was used to detect the expression level of α-SMA and FAP in 20 cases of normal cervical tissues, 20 cases of （ CIN）, 98 cases of invasive cervical cancer. The positive expressions of α-SMA and FAP were compared among the 3 groups of tissues, and the correlation between the positive expression rates of α-SMA and FAP and different clinicopathological characteristics was also analyzed. Results α-SMA and FAP lesions did not express in normal cervix and CIN, however, α-SMA and FAP were expressed in the cytoplasm of tumor interstitial fibroblast of invasive cervical cancer, FAP showed positive expression in a few tumor cells. The positive expression rate of α-SMA in cervical cancer stroma was 82.7% （81/98） , which was related to clinical stage, pathological grade and lymph node metastasis （ P 〈 0. 05 ）, the positive expression rate of FAP in cervical cancer stroma was 79. 6% （78/98）, which was related to clinical stage, pathological grading, and lymph node metastasis （ P 〈 0. 05 ）. α-SMA and FAP expression were not associated with age, tumor size, statistically meaningless （ P 〉 0. 05 ）. The expressions of α-SMA and FAP in invasive cervical carcino ma specimens showed consistency on expression area and intensity. The linear correlation analysis showed there was a linear correlation of α-SMA and FAP （ r = 0. 829, P = 0. 000）. Conclusion α-SMA and FAP may play a key role in the growth, invasion and metastasis of invasive cervical cancer, which can be used as reliable indicators to predict the malignant biological behavior of cervical carcinoma and prognosis.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2015年第11期1248-1252,共5页
Chinese Journal of Clinical and Experimental Pathology
