摘要
目的：建立同时测定补乌酊中4种补骨脂类成分含量的方法。方法：采用高效液相色谱法。色谱柱为Dikma Diamonsil C18,流动相为乙腈-0.2%冰醋酸（梯度洗脱）,流速为1 ml/min,检测波长为246 nm,柱温为30℃,进样量为15μl。结果：异补骨脂素、补骨脂甲素、补骨脂定、补骨脂乙素的检测质量浓度线性范围分别为13.00~208、26.00~416、24.50~392、37.88~606μg/ml（r≥0.999 6）;精密度、稳定性、重复性试验的RSD〈2.00%;加样回收率分别为95.22%~97.23%、100.24%~104.64%、102.28%~104.39%、97.68%~100.17%,RSD分别为0.87%、1.62%、1.47%、0.97%（n=6）。结论：该方法操作简便、重复性好,可作为控制补乌酊质量的方法。
OBJECTIVE：To establish a method for simultaneous determination of 4 psoralen compounds in Buwu tincture.METHODS：HPLC was performed on the column of Dikma Diamonsil C18 with mobile phase of acetonitrile-0.2% Acetic acid by gradient elution at flow rate of 1 ml/min,detection wavelength was 246 nm,column temperature was 30 ℃,and the injection volume was 15 μl. RESULTS：The linear range was 13.00-208 μg/ml for angelicin,26.00-416 μg/ml for bavachin,24.50-392 μg/ml for psoralidin and 37.88-606 μg/ml for isobavachalcone,respectively（r≥0.999 6）;RSDs of precision,stability and reproducibility tests were less than 2.00%;recoveries were 95.22%-97.23%（RSD=0.87%,n=6）,100.24%-104.64%（RSD=1.62%,n=6）,102.28%-104.39%（RSD=1.47%,n=6）and 97.68%-100.17%（RSD=0.97%,n=6）,respectively. CONCLUSIONS：The method is simple and reproducible,and can be used for the quality control of Buwu tincture.
出处
《中国药房》
CAS
北大核心
2015年第33期4734-4736,共3页
China Pharmacy
