摘要
以负调控禾本科植物分蘖的关键基因TB1为研究对象,根据水稻、玉米、高粱TB1同源基因保守序列设计引物,使用RT-PCR方法和RACE技术从甘蔗茎尖处克隆到该基因的同源基因Sc TB1。Sc TB1 c DNA全长1 668 bp,包含274 bp的5′UTR,1 149 bp的CDS和245 bp的3′UTR。生物信息学分析表明该基因可编码382个氨基酸残基,编码产物含有保守的SP区、TCP区和R区,属于TCP家族转录因子。其分子量为40.7 ku,理论等电点为8.55,蛋白亚细胞定位预测其主要定位于细胞质。系统进化分析表明Sc TB1属于CYC/TB1亚族蛋白,与高粱TB1和玉米TB1聚为一个亚类。根据序列的保守性和预测结果可推测Sc TB1很可能也参与了甘蔗分蘖性状的调控。
In this study, TB1 homolog of sugarcane was cloned from the stem apex by using RT-PCR and RACE method referring to the conserved sequence of TB1 homologs in rice, maize and sorghum. The c DNA length of Sc TB1 is 1 668 bp, which contains 274 bp 5′ UTR, 1 149 bp CDS and 245 bp 3′ UTR. Bioinformatics analysis showed that Sc TB1 belonged to the TCP family of transcription factors, which is composed of 382 amino acid residues and contains three conservative domains（SP, TCP and R domains）. The molecular weight and theoretical isoelectric point of Sc TB1 was 40.7 ku and 8.55, respectively, and the gene was likely located in cytoplasm.Phylogenetic tree analysis indicates that Sc TB1 belonged to CYC/TB1 subfamily and had a very close relationship with TB1 homologs of maize and sorghum. Based on the sequence conservation, it is suggested that Sc TB1 may be required for the regulation of tillering trait. This study provides important information for further research on the functional analysis and regulating mechanisms of Sc TB1 in future.
出处
《热带作物学报》
CSCD
北大核心
2015年第11期1978-1985,共8页
Chinese Journal of Tropical Crops
基金
国家自然科学基金(No.31360359)
云南省中青年学术技术带头人后备人才(No.2014HB038)
云南省攻关项目(No.2012BB014)
经济作物种质资源发掘与创新利用(No.2013BAD01B03-15)
云南省应用基础研究计划青年项目(No.2015FD063)
