摘要
目的探讨洛阳地区感染的幽门螺杆菌(H.pylori)菌株的空泡毒素基因(vacuolatingcytotoxingene,VacA)及其亚型的临床分布特征,找出该地区VacA优势基因型并探讨其与胃肠疾病的关系。方法收集“c尿素呼气试验检测阳性,胃镜及病理诊断为慢性胃炎、消化性溃疡、十二指肠球炎、胃癌、胃息肉、反流性食管炎、其他等546例患者(男294例,女252例,年龄10-79岁),采用酶联免疫吸附试验(ELISA法)检测VacA。再将VacA检测阳性者进一步检测VacA亚型:VacA—S1a、VacA—slb、VacA—s2、VacA—M1、VacA—M2,并进行统计学处理。结果洛阳地区546例H.pylori感染患者中,VacA的总阳性率为48.35%,市区内VacA的阳性率为40.00%,市区外县VacA的阳性率为50.70%,三者相比差异无统计学意义(P〉0.05)。VacA亚型S1a、S1b、S2、M1、M2的阳性率分别为76.52%、20.45%、0.76%、21.97%、72.24%,s2很少见。VacA基因亚型嵌合体S1a/M1、S1a/M2、S1b/M1、S1b/M2的阳性率分别为12.88%、47.73%、3.79%、12.88%,未发现s2嵌合体。s区未分型6例,M区未分型10例。VacA亚型及亚型嵌合体在不同胃肠疾病之间分布差异无统计学意义(P〉0.05)。结论洛阳地区感染npylori菌株的VacA的阳性率为48.35%;优势基因亚型为S1a/M2;VacA亚型及亚型嵌合体在不同胃肠疾病之间分布差异无统计学意义。
Objective To investigate the clinical distribution characteristics of infection of Helicobacter pylori (H. py- lori) strains of cavitating toxin gene and its subtype in Luoyang and find out the predominant genotype of VacA and discuss its relationship with gastrointestinal diseases. Methods Five hundred and forty-six patients whose result for 14C urea breath test were positive, and whose gastroscopy and pathological diagnosis were chronic gastritis, peptic ulcers, duodenal bulb inflammation, the stomach cancer, gastric polyps, reflux esophagitis and so on (male 294 cases, female 252 cases, the age range was from 10 to 79 years old) were collected, enzyme-linked immunosorbent assay (ELISA) was used to de- tect VacA. The VacA subtypes from the positive VacA test results included VacA-Sla, VacA-Slb, VacA-S2, VacA-M1, VacA-M2 were detected. Results In the 546 cases of H. pylori infection in Luoyang, the total positive rate of VacA was 48.35% , and the positive rate of VacA in downtown was 40.00% , while the total positive rate of VacA outside the city was 50.70%. There was no significant difference among them. The positive rates of vacA subtype Sla, Slb, $2, M1, M2 were 76.52% , 20.45% , 0.76% , 21.97% , 72.24% , respectively. $2 was rare. The positive rates of VacA gene subtype chimeras Sla/M1, Sla/M2, Slb/M1, Slb/M2 were 12.88% , 47.73% , 3.79% , 12.88% , respectively. And the $2 chimeras was not found. Six cases in S area and 10 cases in M area were nontypeable. There was no significant difference among the distributions of vacA subtypes and subtype of chimeras in different gastrointestinal diseases. Conclusion The positive rate in Luoyang of the infection of H. pylori strains of cavitating toxin genes is 48.35% , and the predominant geno- type is Sla/M2. There is no significant difference among the distributions of VacA subtypes and subtype of chimeras in different gastrointestinal diseases.
出处
《胃肠病学和肝病学杂志》
CAS
2015年第11期1384-1386,共3页
Chinese Journal of Gastroenterology and Hepatology