摘要
目的探讨早期低剂量接触全氟辛烷磺酸盐(perfluorooctane sulfonate,PFOS)对大鼠生殖功能和生长发育的影响及S-腺苷L-甲硫氨酸(S adenosyl L methionine,SAM)和5-杂氯脱氧胞嘧啶(decitabine,DAC)对其的干预作用。方法将40只健康出生5 d(PND5)SPF级SD大鼠随机分为4组,每组10只,雌雄各半,分别为对照(0.2%Tween-80生理盐水)组、PFOS染毒组、SAM干预组和DAC干预组。PFOS染毒组、SAM干预组和DAC干预组分别染毒5 mg/kg的PFOS;SAM干预组和DAC干预组分别同时给与16 mg/kg的SAM和0.15 mg/kg的DAC干预;采用颈部皮下注射方式进行染毒,每天1次,28 d后,每3 d 1次,持续染毒至出生后60 d(PND60)。每天观察F0代大鼠的一般情况并测定体重。待F0代大鼠成年后,将雌、雄大鼠按1∶1合笼交配。待F1代大鼠成年后,各组选取6只大鼠,以雌∶雄为2∶1进行合笼,孕期和哺乳期(PND28)母鼠的染毒方法同前。观察各代大鼠的生育能力和仔鼠的发育情况,并观察F1代大鼠睾丸组织形态结构变化。结果与对照组比较,PFOS组、SAM干预组和DAC干预组F0代母鼠的受孕率均较低,死产率较高,存活率较低;PFOS组和DAC干预组F0代母鼠的畸胎率和产后死亡率均较高,差异均有统计学意义(P<0.01,P<0.05)。与PFOS染毒组比较,SAM干预组F0代母鼠的受孕率、存活率较高,死产率、畸胎率较低;DAC干预组F0代母鼠的死产率、存活率较低,产后死亡率较高,差异均有统计学意义(P<0.01,P<0.05)。与对照组比较,PFOS染毒组和DAC干预组F1代仔鼠出生体重均较低,毛发长出时间均延长,差异有统计学意义(P<0.05,P<0.01);PFOS染毒组和SAM干预组、DAC干预组F1代仔鼠的开眼时间和自主采食出现时间均延长,差异有统计学意义(P<0.05),而耳廓伸展时间无明显改变。与PFOS染毒组比较,SAM干预组F1代仔鼠的出生体重较高,开眼时间和自主采食出现时间均缩短,差异有统计学意义(P<0.05,P<0.01);DAC干预组F1代仔鼠�
Objective To detect the potential effects of S-adenosyl L-methionine（SAM） and decitabine（DAC） on the reproductive and developmental toxicity induced by perfluorooctane sulfonate（PFOS）. Methods Forty SD of PND5 SD rats were randomly divided into control,PFOS,PFOS ＋SAM and PFOS ＋DAC groups, ten in each group, half male and female. The rats were subcutaneously injected on neck with PFOS（5 mg/kg body weight） in PFOS, PFOS ＋SAM and PFOS ＋DAC group, with equivalent of 0.2% Tween-80 saline in control group, co-treated with SAM（16 mg/kg body weight） or DAC（0.15 mg/kg body weight） in PFOS＋SAM or PFOS＋DAC group, once a day for 28 d, then once three days till PND60. The F0 adult rats（male ∶female=1∶1） were put into a cage for mating. The adult F1 rats（female∶male= 2∶1）,six rats in each group,were put into a cage for mating. The treatments of PFOS and SAM or DAC to maternal rats during pregnancy and lactation were the same as before. The fertility of maternal rats and development of neonates were detected. The morphology of male testis was also observed. Results Compared with the control group,the pregnancy rates and viability were significantly lower and the mortality rates were higher in PFOS, PFOS＋SAM and PFOS＋DAC groups. The teras rates and postpartum mortality were higher in PFOS and PFOS＋DAC groups. Compared with PFOS group, the pregnancy rates and viability of neonates in F0 rats were higher,in PFOS＋SAM group.The birth weights of rats（F1） in PFOS and PFOS＋DAC group were significantly lower, the time of hair appear,opening of eyes and independent foraging were later than those in control rats,whereas,those of which were quite the same as in PFOS ＋SAM group and control group. After normal feeding for two months, there was offspring of two male and four female in PFOS group,three male and four female in PFOS＋DAC group, both of which female rats were infertility, however, there were neonates of four male and four female in PFOS ＋SAM group, in which fe
出处
《环境与健康杂志》
CAS
北大核心
2015年第8期672-676,F0003,共6页
Journal of Environment and Health
基金
国家自然科学基金(81060231
81160338
81301683)
