摘要
目的观察益气养阴方对非酒精性脂肪性肝病(NAFLD)大鼠胰岛素抵抗(IR)的影响,探讨其作用机制。方法采用饲喂高脂饲料建立大鼠NAFLD模型。实验大鼠随机分为正常组、模型组、阳性药组、益气养阴方组,分别给予蒸馏水、吡格列酮、益气养阴方灌胃,连续8周。进行口服葡萄糖耐量试验(OGTT),HE染色观察肝脏脂肪空泡样改变,放免法检测血清胰岛素含量,GPO-PAP法检测血清三酰甘油(TG)含量。结果模型组大鼠肝组织出现脂肪堆积导致的空泡样病理损伤,阳性药组、益气养阴方组与模型组比较上述病理损伤明显减轻;OGTT显示口服葡萄糖2 h后,模型组血糖明显高于正常组;与模型组比较,阳性药组、益气养阴方组血糖、血清胰岛素水平和TG含量明显降低(P<0.05)。结论益气养阴方可有效防治NAFLD大鼠IR,其作用机制可能是通过抑制模型大鼠异常升高的TG和胰岛素水平,纠正机体糖脂代谢紊乱,改善其IR。
Objective To observe the effects of Yiqi Yangyin Formula on insulin resistance(IR) of rats with non-alcoholic fatty liver disease(NAFLD). Methods High-fat diet was fed to establish NAFLD rat models. Experimental rats were randomly divided into normal group, model group, positive medicine group, and Yiqi Yangyin Formula group. The groups received distilled water, pioglitazone and Yiqi Yangyin Formula respectively for gavage for 8 weeks. OGTT was conducted;HE dyeing was used to detect the fat spongiform in liver;Radioimmunoassay was used to detect insulin content in serum;GPO-PAP was used to detect TG content in serum. Results HE dyeing results showed that vacuolar pathological injury caused by fat accumulation in liver tissue occurred in the liver of rats in the model group, while pathology injury decreased significantly in positive medicine group and Yiqi Yangyin Formula group. OGTT results showed that after 2 h of oral administration, the blood glucose in the model group was greatly higher than the normal group. Compared with the model group, the blood glucose insulin level and TG content in serum decreased significantly in the positive medicine group and Yiqi Yangyin Formula group(P <0.05). Conclusion Yiqi Yangyin Formula can effectively prevent and treat IR of rats with NAFLD. Its mechanism may be achieved through decreasing the abnormal insulin and TG, regulating glucose metabolism disorder.
出处
《中国中医药信息杂志》
CAS
CSCD
2015年第12期47-49,共3页
Chinese Journal of Information on Traditional Chinese Medicine
基金
国家中医药管理局中医药重点学科(2010年)
