摘要
为探讨桦褐孔菌乙醇提取物对植物病原真菌的抑制作用,采用醇提法获得桦褐孔菌乙醇提取物,通过观察稻瘟病的稻梨孢菌孢子萌发和菌丝形态结构及小麦根腐平脐孺孢和番茄灰霉灰葡萄球菌的平板对峙实验结果,研究桦褐孔菌乙醇提取物抗病原真菌活性。结果表明,用醇提法获得的桦褐孔菌乙醇提取物得率为7.35%;乙醇提取物浓度为3.125 mg·m L-1时,对稻瘟病菌孢子萌发的抑制率为94%,浓度高于6.25 mg·m L-1时,抑制率均为100%;在乙醇提取物的作用下,稻瘟病菌菌丝出现粗细不均、结构松散、细胞壁加厚等现象;小麦根腐平脐孺孢和番茄灰霉灰葡萄球菌的平板对峙实验均出现较大的抑菌圈。本研究为深入探讨桦褐孔菌乙醇提取物抗真菌病害的机制及其应用奠定了基础。
In order to investigate the antifungal activity of ethanol extract of Inonotus obliquus for plant pathogenic fungi,the ethanol extract was obtained by ethanol precipitation method and the antifungal activity was explored by spore germination and hyphal structure variation of Pyricularia oryzae, and plate confrontation experiments of Bipolaris sorokiniana and Botrytis cinerea. Results showed the extraction rate of Inonotus obliquus ethanol extract by ethanol precipitation method was 7. 35%. The inhibition rate of spore germination was 94% for Pyricularia oryzae at the extract concentration of 3. 125mg·mL^- 1,and the inhibition rate was increased to 100% when the concentrations was higher than 6. 25mg·mL^- 1. The hyphal structure of Pyricularia oryzae became unsymmetrical and incompact after the treatment of the extracts and its cell wall was also thickened. The obvious inhibition zones appeared on the plates in confrontation experiments of Bipolaris sorokiniana and Botrytis cinerea. Here we establishes the foundation for deep research on mechanism of antifungal activity of ethanol extract of Inonotus obliquus and its application.
出处
《核农学报》
CAS
CSCD
北大核心
2015年第11期2239-2245,共7页
Journal of Nuclear Agricultural Sciences
基金
黑龙江省微生物菌种资源共享平台建设项目(KT05A400-1)
关键词
桦褐孔菌
乙醇提取物
植物病原真菌
抗真菌活性
Inonotus obliquus
ethanol extract
plant pathogenic fungi
antifungal activity
