摘要
实验建立一种利用硅胶固相萃取-高效液相色谱来检测油炸肉制品中丙烯酰胺的含量的方法。采用硅胶作为萃取材料对样品提取液进行净化，利用高效液相色谱进行检测。结果表明：检测波长为205 nm，流动相为甲醇-水（5∶95，V/V），流速为0.8 mL/min，方法加标回收率为73.5%～80.8%，相对标准偏差≤5.54%，最低检出限为10 ng/mL。该方法简便、可靠，满足油炸肉制品中丙烯酰胺的检测要求。
This paper presents a silica gel solid phase extraction-high performance liquid chromatography （HPLC） method to detect the content of acrylamide in fried meat products. The extract was cleaned up by solid phase extraction using silica gel as the adsorbent prior to being analyzed by HPLC. The detection wavelength was set at 205 nm. The separation was performed using a Kromisil C18 （250 mm × 4.6 mm, 5 μm）. The mobile phase used was a 5：95 （V/V）mixture of methanol and water at a lfow rate of 0.8 mL/min. Recoveries of the proposed method for spiked samples ranged from 73.5% to 80.8%, with relative standard deviation （RSD） no greater than 5.54% （n= 5）. The limit of detection was 10 ng/mL （RS/N= 3）. This method is simple and reliable, and can be used for the detection of acrylamide in fried meat products.
出处
《肉类研究》
北大核心
2015年第8期28-30,共3页
Meat Research
基金
2013年烟台市科技支撑计划项目(2013NC333)
关键词
油炸肉制品
硅胶
固相萃取
高效液相色谱
fried meat products
silica gel
solid phase extraction
high performance liquid chromatography （HPLC）
