摘要
目的 探讨孕妇血浆和胎盘组织中的血栓调节蛋白（TM）表达与早发型重度子痫前期发病的关系.方法 选择2012年6月至2014年2月在徐州医学院附属徐州市妇幼保健院住院分娩的重度子痫前期孕妇60例.其中,早发型重度子痫前期孕妇30例为早发型组,晚发型重度子痫前期孕妇30例为晚发型组,选择同期健康孕妇并根据其孕周不同分为早发型对照组（孕周28-33周＋6）23例和晚发型对照组（孕周≥34周）30例.采用ELISA法检测各组孕妇血浆中TM的水平,免疫组化SP法检测胎盘组织中TM蛋白的表达,逆转录（RT）-PCR技术检测胎盘组织中TM mRNA的表达.结果（1）早发型组孕妇血浆TM水平为（90.8 ± 6.9）μg/L,晚发型组为（87.5 ± 7.0）μg/L,早发型对照组为（37.7±2.3）μg/L,晚发型对照组为（37.7±2.5）μg/L.早发型组孕妇血浆TM水平分别高于其他3组,但早发型组与晚发型组比较,差异无统计学意义（P〉0.05）;早发型组与早发型对照组比较,差异有统计学意义（P〈0.05）;晚发型组高于晚发型对照组,差异有统计学意义（P〈0.05）.（2）TM蛋白主要定位于胎盘合体滋养细胞和血管内皮细胞的胞膜及胞质中,早发型组胎盘组织中TM蛋白的阳性表达率（47%,14/30）显著低于晚发型组（90%,27/30）、早发型对照组（91%,21/23）及晚发型对照组（93%,28/30）,分别比较,差异均有统计学意义（P〈0.05）;晚发型组与晚发型对照组比较,差异无统计学意义（P〉0.05）;早发型对照组与晚发型对照组比较,差异无统计学意义（P〉0.05）.（3）早发型组胎盘组织中TM mRNA的表达水平为0.14±0.06,晚发型组为0.89±0.23,早发型对照组为0.88±0.22,晚发型对照组为0.93±0.19,早发型组胎盘组织中TM mRNA表达水平显著低于对其他3组,差异均有统计学意义（P〈0.05）;晚发型组与晚发型对照组比较,差异无统计学意义（P〉0.05）;早发型对照组与晚发�
Objective To explore the thrombomodulin（TM） expreesion levels changes in plasma and placenta in patients with early onset severe preeclampsia. Methods Sixty cases of severe preeclampsia women who delivered in the affiliated Xuzhou Maternity and Child Health Care Hospital of Xuzhou Medical College were enrolled in the study from June 2012 to February 2014, including 30 patients with early onset severe preeclampsia （early onset group）, and 30 patients with late onset severe preeclampsia （late onset group）. Healthy pregnant women were divided into two control groups according to gestational weeks at delivery： early control group （ n=23, at 28-33＋6 weeks）, and late control group （n=30, delivered after 34 weeks）. ELISA was used to detect the levels of TM in plasma. Immunohistochemistry SP was applied to detect the TM protein expression on placenta. TM mRNA was determined by reverse transcription polymerase chain reaction （RT）-PCR technique. Results （1） TM level in plasma in early onset group and late onset group were （90.8±6.9） and （87.5±7.0）μg/L, and TM level in plasma in early control group and late control group were （37.7 ± 2.3） and （37.7 ± 2.5）μg/L. Plasma TM level in early onset group was higher than that in late onset group, early control group and late control group. The TM level had no statistically significant compare of early-onset group to late onset group.（P〉0.05）. The plasma TM level in early onset group was significantly higher than that in early control group （P〈0.05）, and the plasma TM level in late onset group was significantly higher than that in late control group （P〈0.05）. （2）TM expressed mainly in the membrane and cytoplasm of placental syncytiotrophoblasts and endothelial cells. The expression of TM protein in early onset group was 47%（14/30）, significantly lower than that in late onset group, early control group and late control group （P〈0.05）, in which the positive rate were 90%（27/30）, 91%（21/23） and
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2015年第10期752-756,共5页
Chinese Journal of Obstetrics and Gynecology
