摘要
目的 对机械通气患者分离出的鲍曼不动杆菌及铜绿假单胞菌进行流行病学调查.方法 收集2012年10至12月北京大学第一医院心外科重症监护室（ICU）11例有创机械通气患者的呼吸道分泌物及血标本,对分离到的鲍曼不动杆菌和铜绿假单胞菌进行纸片法抗菌药物敏感试验,并通过脉冲场凝胶电泳（PFGE） DNA指纹图技术对菌株进行比对,确定菌株的同源性,同时结合临床资料进行分析.结果 共分离出7株鲍曼不动杆菌和8株铜绿假单胞菌.7株鲍曼不动杆菌分别来自不同克隆,提示为特定位置的特定菌株簇,应为散发感染或定植菌株;8株铜绿假单胞菌中,部分菌株具有高度同源性,为院内获得性感染,确认存在院内感染的交叉传播.结论 心外科ICU未发生鲍曼不动杆菌及铜绿假单胞菌的院内感染暴发流行,但存在铜绿假单胞菌小范围交叉感染.
Objective To investigate the molecular epidemiology of acinetobacter baumannii and pseudomonas aeruginosa isolated from clinical specimens from patients on mechanical ventilation.Methods From October to December , 2012, acinetobacter baumannii and pseudomonas aeruginosa strains were isolated from patients on mechanical ventilation.Antibiotic susceptibility data of all strains was collected.Homology of the strains was analyzed by the methods of pulsed field gel electrophoresis （PFGE） genotype, meanwhile , the clinical data was also analyzed.Results Of the 15 pathogens isolated from patients on mechanical ventilation , 7 acinetobacter baumannii strains had different genotypes , indicating specific clustering in a specific location , so acinetobacter baumannii should be a scattered infection or colonized strains.Parts of 8 pseudomonas aeruginosa strains came from the same clone and confirm the existence of cross-infection of nosocomial infections.Conclusion The result shows that nosocomial infection outbreak doesn＇t occur , there is a small range of cross-infection of pseudomonas aeruginosa.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2015年第40期3269-3272,共4页
National Medical Journal of China
关键词
鲍曼不动杆菌
铜绿假单胞菌
流行病学
脉冲场凝胶电泳
Acinetobacter baumannii
Pseudomonas aeruginosa
Epidemiology
Pulsed field gel electrophoresis
