摘要
目的研究Dermokine(DK)-βmRNA在结肠癌组织中的水平和临床价值。方法采用RT-PCR法检测DK-βmRNA相对水平,对结肠癌患者、结肠息肉、溃疡性结肠炎患者以及健康对照组DK-βmRNA相对水平进行比较,对结肠癌不同临床参数间DK-βmRNA相对水平进行比较,并进行统计学分析。结果结肠癌患者DK-βmRNA表达比结肠息肉、溃疡性结肠炎患者以及健康对照组明显升高,差异有统计学意义(P<0.01);但与患者年龄、性别、肿瘤的位置、大小无相关性,在低分化腺癌、Dukes分期B期、有淋巴结转移的患者中DK-βmRNA表达更明显(P<0.05)。结论结肠癌DK-β表达与结肠癌患者黏膜上皮细胞受损密切相关,B期DK-β表达呈显著升高,因此结肠组织DK-β水平检测可作为结肠癌早期诊断的指标,对临床结肠癌早期诊断有一定价值。
Objective To investigate the level of dermokine-β（DK-β）mRNA in colon cancer tissue and its clinical value.Methods The RT-PCR method was used to detect DK-βmRNA relative level.The DK-βmRNA relative levels were compared among the patients with colon cancer,colonic polyps and ulcerative colitis and normal control group.The DK-βmRNA relative levels were compared among different clinical parameters in colon carcinoma.The statistical awalysis was performed.Results The expression of DK-βmRNA in the patients with colon cancer was significantly higher than that in the patients with colon polyps,ulcerative colitis and normal control group,the difference was statistically significant（P〈0.01）.But the DK-βmRNA expression had no correlation with age,gender,tumor location and size.The DK-βmRNA expression in the low differentiation adenocarcinoma,Dukes B stage and lymph node metastasis was more significant（P〈0.05）.Conclusion The DK-βexpression in colon cancer is closely associated with mucosal epithelial cell damage.The DK-βexpression in the stage B is significantly increased.Therefore,the DK-βlevel detection in colon tissue may be used as an index of early diagnosis of colon cancer,which has a certain value for early diagnosis of colon cancer.
出处
《检验医学与临床》
CAS
2015年第21期3137-3139,共3页
Laboratory Medicine and Clinic
基金
湖北省自然科学基金项目(2013CFB486)
湖北省孝感市科技局基金支持项目(2011XXF042)
