摘要
传统的羽绒品种定量检测方法为显微镜检测法,费时长且依赖检测人员的经验。本研究建立了基于实时荧光PCR技术的鹅鸭混合绒定量检测方法。通过设计针对鹅、鸭线粒体DNA序列的特异性引物和Taq Man探针,且两组引物探针具有几近相同的扩增效率,建立羽绒品种检测的定量标准曲线(R2>0.99)。以不同产地的鹅鸭混合绒验证该方法,均得到较好结果,证明线粒体拷贝数对定量结果基本没有影响。该方法有望用于羽绒品种的大批量快速定量检测。
In this project, a quantitative method based on real-time PCR technique was developed to determine the weight of goose down and duck down in down products; Two sets of primers and TaqMan probes that can react specifically to goose and duck mitochondrial DNA were designed, and they exert ahnost the same amplification rate. The two systems were used to establish the quantitative standard curve of down species determination（R^2〉0.99）. Goose and duck down blends collected from different producing areas were employed to verify the method, and satisfactory results were obtained, which proved that the copy number of mitochondrion has little impact on quantifying results. The method can be applied to quick and large-batch quantitative identification of down species in down products.
出处
《纺织导报》
CAS
北大核心
2015年第11期96-98,共3页
China Textile Leader
基金
上海出入境检验检疫局科研项目(HK007-2014)
关键词
鹅绒
实时荧光PCR
物种鉴定
定量分析
goose down
real-time PCR
identification of species
quantitative analysis
