摘要
目的观察大蒜素对D-半乳糖致衰老大鼠脑部衰老的影响,并从氧化应激通路入手探讨其中可能的机制。方法取50只雄性SD大鼠作为研究对象,随机分为5组(n=10):正常组(C组)、D-半乳糖组(D组)和大蒜素组,其中大蒜素组按剂量分为低剂量组(L组)、中剂量组(M组)和高剂量组(H组)。D、L、M、H组按大鼠体重以125mg/kg的剂量皮下注射D-半乳糖生理盐水溶液,C组皮下注射同体积生理盐水对照,每日8:00注射1次。同时,L、M和H组分别腹腔注射10、20和40mg/kg大蒜素注射液,C组和D组以同体积生理盐水作对照处理,每日20:00注射1次,所有大鼠均持续注射6周。通过行为学实验,观察各组大鼠学习记忆能力。处死后取各组大鼠脑组织:检测丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性及总抗氧化力(T-AOC);另取部分脑组织:固定包埋后石蜡切片,采用免疫组织化学法观察海马区凋亡相关蛋白Bax和Bcl-2的表达。结果行为学实验结果显示,与C组比较,D组大鼠学习记忆能力明显下降,而大蒜素干预的各组大鼠记忆能力有所增强。与C组比较,D组大鼠脑组织匀浆中MDA含量升高(P<0.05),SOD活性下降(P<0.05),T-AOC下降(P<0.05),大蒜素干预的各组大鼠较D组MDA含量下降(P<0.05),SOD活性增强(P<0.05),T-AOC上升(P<0.05),且呈现一定的剂量效应关系。免疫组化结果显示,与C组比较,D组Bax表达明显增加(P<0.05),Bcl-2表达明显减弱(P<0.05),大蒜素干预的L、M、H组大鼠Bax表达较D组呈减弱趋势,Bcl-2表达较D组呈增强趋势,但结果并无统计学意义。结论大蒜素可改善D-半乳糖致衰老大鼠的学习记忆能力,提高脑组织匀浆中SOD活性,降低MDA含量,增强脑组织总抗氧化力,并减轻衰老大鼠脑海马区细胞凋亡。
Objective To investigate the effects of allicin on the brain of D-galactose induced aging rats, and explore the possible mechanism of the effect through studying oxidative stress pathway. Methods Fifty male SD rats were randomly divided into 5 groups（10 each）： control group（Group C）, D-galactose group（Group D）, and allicin groups with different allicin dosage, i.e. low-dose allicin group（Group L）, medium-dose allicin group（Group M） and high-dose allicin group（Group H）. Rats in group C received subcutaneous injection of 0.5ml normal saline, and those in groups D, L, M and H were given injection of 125mg/kg D-galactose for 6 weeks（one time every day at 8：00 am）. Meanwhile, rats in groups L, M and H were intraperitoneally injected with allicin in the doses of 10, 20 and 40mg/kg, respectively, while those in groups C and D were injected with the same volume of normal saline as control for 6 weeks（one time every day at 8：00 pm）. The learning and memory ability of the rats were observed by ethological examination, and animals were then sacrificed and the brain tissues were acquired. The malondialdehyde（MDA） content, superoxide dismutase（SOD） activity, and the total antioxidant capacity（T-AOC） in the homogenate of brain tissue were measured. Another part of brain tissue was examined under the microscope, and the expression of the apoptosis related proteins in hippocampus was examined. Bax and Bcl-2 expressions were also determined by immunohistochemistry. Results Ethological examination indicated that, compared with that in group C, the learning and memory ability were attenuated obviously in group D（P0.05）, while the memory ability increased in the 3 allicin groups（P0.05）. Compared with that in group C, the MDA content increased（P0.05）, SOD activity lowered（P0.05）, and T-AOC decreased（P0.05） in group D. Compared with that in group D, the MDA content decreased（P0.05）, and SOD activity and A-TOC increased in the 3 allicin groups（P0.05） with a c
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2015年第11期880-884,共5页
Medical Journal of Chinese People's Liberation Army
基金
国家自然科学基金面上项目(81372793)
吉林省"十二五"科学技术研究项目(2014552)~~
关键词
大蒜素
衰老
脑
氧化应激
凋亡
allicin
aging
brain
oxidative stress
apoptosis
