摘要
目的探讨血管内皮生长因子(VEGF)和Cleaved Caspase-3在宫颈鳞癌组织中的表达及临床意义。方法应用免疫组织化学法、荧光实时定量聚合酶链反应(PCR)和Western blot法分别检测在46例慢性宫颈炎和52例宫颈鳞癌组织中VEGF和Cleaved Caspase-3的表达情况。结果VEGF和Cleaved Caspase-3在不同组织中的相对表达量均不同,慢性宫颈炎组的VEGF基因和蛋白表达量明显低于宫颈鳞癌组(P<0.01)。Cleaved Caspase-3基因和蛋白表达呈相反趋势。VEGF和Cleaved Caspase-3表达均与淋巴结转移、国际妇产科联盟(FIGO)分期有关,差异均有统计学意义(P<0.05)。结论在宫颈鳞癌组织中,VEGF过表达,但Cleaved Caspase-3呈低表达,两者均参与了宫颈鳞癌的发生发展,其联合检测有助于判断患者的预后。
Objective To investigate the expression of vascular endothlial growth factor（ VEGF）and Cleaved Caspase-3 in cervical carcinoma and their clinical significance. Methods The expression of VEGF and Cleaved Caspase-3 were detected by immunohisochemisty,real-time polymerase chain reaction（ PCR） and Western blot in 46 patients of chronic cervicitis and 53 patients of malignant lesion. Results Relative expression of vascluar endothial growth factor（ VEGF） were different in different cervical carcinomas. Expression of VEGF gene and protein in uterine fibroids and ovarian cyst were significantly lower than that of cervical cancer group（ P 0. 01）,but the expression of Cleaved Caspase-3 gene and protein had the opposite trend. Cleaved Caspase-3 and VEGF expression were related to lymph node metastasis and Federation International of Gynecology and Obstetrics（ FIGO） stage,and the differences were statistically significant（ P 0. 05）. Conclusions VEGF is over-expression and Cleaved Caspase-3 is low-expression in cervical aquamous cell carcinoma tissue,which are both involved in the occurrence and development of cervical aquamous cell carcinoma. Therefore,the detection of VEGF and Cleaved Caspase-3 is helpful for the prognosis of patients.
出处
《中国肿瘤临床与康复》
2015年第11期1306-1310,共5页
Chinese Journal of Clinical Oncology and Rehabilitation
基金
国际和平妇幼保健院院级课题资助
No09411955100
