摘要
目的建立快速酸浸取石墨炉原子吸收法测定全血微量铅、镉、铬的方法。方法血样加5%硝酸后采用振荡、高速离心法进行样品前处理,以2%磷酸二氢铵与0.2%硝酸镁混合液为基改剂,塞曼扣背景及标准加入法对样品中的微量铅、镉、铬进行测定。结果铅、镉和铬分别在5μg/L^60μg/L、0.5μg/L^6μg/L和2μg/L^20μg/L时具有良好的线性,相关系数均>0.999,RSD为2.31%~5.29%,回收率为95.3%~104.0%。采用本法对美国Wisconsin State Laboratory of Hygiene全球血铅比对样品进行方法验证,所测结果均在标准参考值内。结论本法具有快速、简便、环保、准确的优点,适合批量同时分析全血中铅、镉、铬。
Objective To establish a method for determination of trace lead,cadmium and chromium in whole blood by graphite furnace atomic absorption apectrometry with acid extraction. Methods The target elements in whole blood were extracted with5% nitric acid and then the extractant was determined by graphite furnace atomic absorption spectrophotometry. The mixture of2% of ammonium dihydrogen phosphate solution and 2% of magnesium nitrate solution was used as matrix modifier,and zeeman background correction and standard addition method were used for quantification. Results A good linear ranges were achieved within 5 μg / L-60 μg / L,0. 5 μg / L-6 μg / L and 2 μg / L-20 μg / L for lead,cadmium and chromium,respectively,with correlation coefficients more than 0. 999. The recoveries were among 95. 3%-104. 0%,and RSD was among 2. 31%-5. 29%. The method was validated using the reference of global blood lead from Wisconsin State Laboratory of Hygiene in the United States,and all the measured results were within the allowed values of the standard references. Conclu sion This method is rapid,convenient,environmentally friendly and accurate,it is suitable for the analysis of lead,cadmium and chromium in whole blood with large quantities samples.
出处
《中国卫生检验杂志》
CAS
2015年第20期3433-3435,共3页
Chinese Journal of Health Laboratory Technology
基金
浙江省微量有毒化学物健康风险评估技术研究重点实验室开放基金项目(2014004)
关键词
血铅
血镉
血铬
石墨炉原子吸收法
Blood lead
Blood cadmium
Blood chromium
Graphite furnace atomic absorption spectrophotometry
