摘要
以早酥梨成熟叶片为研究对象,考察超声时间、料液比、溶剂种类3个因素对梨叶多酚提取效果的影响,以优化梨叶片多酚提取工艺,并采用超高效液相色谱-光电二极管阵列技术,测定梨叶片多酚物质组成和含量。结果表明：梨叶片中熊果苷最佳提取条件是：采用70%甲醇溶液提取溶剂、超声（超声功率500 W）辅助提取2次、每次超声时间30 min、料液比1∶150（g/m L）;梨叶片中咖啡酰奎宁酸类和黄酮醇类的最佳提取条件均是：超声（超声功率500 W）辅助提取2次、每次超声时间25 min、采用70%甲醇溶液提取溶剂、料液比1∶125（g/m L）。在优化的提取条件下,梨叶中共检测出10种多酚物质,其中熊果苷含量最高为7.934--36.854 mg/g,被检测出的多酚物质总含量高达17.852--61.149 mg/g,因此梨叶片可作为开发化妆品、保健品和药品的优质天然材料。
The ultrasonic-assisted extraction of polyphenols from mature leaves of Zaosu pear was optimized by investigating the effects of ultrasonic time, solid-to-solvent ratio and solvent type on extraction efficiency. The components and contents of pear leaf polyphenols were determined by ultra performance liquid chromatography（UPLC） coupled with photodiode array detector（PDA）. The results showed that the optimal conditions for arbutin extraction from pear leaves were found to be 30 min extraction carried out twice using 70% aqueous ethanol as the extraction solvent with a solid-to-solvent ratio of 1：150（g/m L） at an ultrasonic power of 500 W, while the optimal conditions for the extraction of both caffeoylquinic acid and flavonols were identical to those for arbutin except for an extraction time of 25 min and a solid-to-solvent ratio of 1：125（g/m L）. Ten polyphenols were detected in pear leaves under the optimized conditions with total contents ranging from 17.852 to 61.149 mg/g, and the contents of arbutin were 7.934–36.854 mg/g. Therefore, pear leaves can be used as an excellent natural material for developing cosmetics, health products and medicines.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2015年第20期32-36,共5页
Food Science
基金
国家现代农业(梨)产业技术体系建设专项(CARS-29-01)
关键词
早酥梨叶
超高效液相色谱
多酚
提取
成分
Zaosu pear leaf
ultra performance liquid chromatography（UPLC）
polyphenols
extraction
components
