摘要
为阐明Sh2甜玉米（Zea mays subsp.saccharata Sturt.）种子萌发过程中关键水解酶对种子贮藏物质利用的作用,以Sh2甜玉米自交系BF109和Q267的种子为材料,对种子萌发2、4、6、8和10 d时贮藏物质的消耗量和利用率以及总淀粉酶和蔗糖合成酶的活性进行了测定,并采用实时荧光定量PCR技术对9个相关基因的表达特性进行了分析。结果表明：种子萌发6 d时2个自交系的单粒种子贮藏物质消耗量大幅度提高,说明种子内的贮藏物质进入快速分解阶段。随萌发时间的延长,2个自交系的单粒种子贮藏物质消耗量逐渐升高,而贮藏物质利用率则呈先升高后降低的趋势;总体上看,自交系BF109的单粒种子贮藏物质消耗量显著高于自交系Q267（P〈0.05）,而其贮藏物质利用率则显著低于后者。随萌发时间的延长,2个自交系种子内的总淀粉酶和蔗糖合成酶活性也总体呈先升高后降低的趋势,并且自交系BF109种子内2种酶活性基本上低于或显著低于自交系Q267。实时荧光定量PCR分析结果表明：在种子萌发2~10 d期间,2个自交系种子中9个α-淀粉酶和蔗糖合成酶相关基因的相对表达量均有较大差异,但7个α-淀粉酶相关基因（包括α-Amy1A、α-Amy2A、α-Amy3A、α-Amy3B、α-Amy3C、α-Amy3D和α-Amy3E）的相对表达量均在萌发前期较高,利于合成α-淀粉酶并参与淀粉水解;α-Amy1A、α-Amy2A、α-Amy3D和Su Sy-2基因的相对表达量在种子萌发2~10 d均较高,而α-Amy3A、α-Amy3B、α-Amy3C、α-Amy3E和Su Sy-3基因的相对表达量仅在种子萌发2 d时较高,说明α-Amy1A、α-Amy2A、α-Amy3D和Su Sy-2基因可能是Sh2甜玉米种子萌发过程中的关键水解酶基因。总体来看,自交系Q267种子中相关基因的相对表达量显著高于自交系BF109,这可能是导致自交系Q267种子中总淀粉酶和蔗糖合成酶活性及贮藏物质利用率较高的主要原因。
In order to clarifying the effect of key hydrolytic enzymes on storage substance utilization in seed of Sh2 sweet corn （Zea mays subsp, saccharata Sturt.） during seed germination process, taking seeds of inbred line BF109 and Q267 of Sh2 sweet corn as materials, consumption and utilization rate of storage substances and activities of total amylase and sucrose synthase were detected when seed germinated for 2,4, 6, 8 and 10 d, and expression characteristics of nine related genes were analyzed by real-time fluorescence quantitative PCR technology. The results show that consumption of storage substances in single seed of two inbred lines increases greatly when seed germinates for 6 d, meaning that storage substances in seed enter fast decomposition stage. With prolonging of germination time, consumption of storage substances in single seed of two inbred lines increases gradually, while utilization rate of storage substances appears the trend of firstly increasing and then decreasing. On the whole, consumption of storage substances in single seed of inbred line BF109 is significantly higher than that of inbred line Q267 （ P〈0.05 ）, while its utilization rate of storage substances is significantly lower than that of the latter. With prolonging of germination time, activities of total amylase and sucrose synthase in seed of two inbred lines also generally appear the trend of firstly increasing and then decreasing, and activities of two enzymes in seed of inbred line BF109 are basically lower or significantly lower than those of inbred line Q267. Analysis result of real-time fluorescence quantitative PCR shows that during seed germination for 2-10 d, there is a great difference in relative expression of nine genes related to α-amylase and sucrose synthase in two inbred line seeds, but relative expressions of seven genes related to a-amylase including α-AmylA, α-Amy2A, α-Amy3A, α-Amy3B, α-Amy3C, α-Amy3D and α-Amy3E all are higher at the early germination stage, which is beneficial to synthetize α-amyla
出处
《植物资源与环境学报》
CAS
CSCD
北大核心
2015年第3期18-24,共7页
Journal of Plant Resources and Environment
基金
国家自然科学基金委员会生命科学部应急管理项目(31440066)
国家自然科学基金青年科学基金资助项目(31101598)
安徽省教育厅自然科学资金项目(KJ2013B79)
安徽科技学院省级科研平台开放课题资助项目(ZRC2013392)
关键词
Sh2甜玉米
种子萌发
贮藏物质利用率
淀粉酶
蔗糖合成酶
基因表达
Sh2 sweet corn （Zea mays subsp, saccharata Sturt.）
seed germination
utilization rate of storage substances
amylase
sucrose synthase
gene expression
