摘要
目的 比较辅助生殖技术（ART）中分裂期胚胎（D3）玻璃化冷冻保存不同时间后复苏胚胎的质量与临床结局。方法 根据分裂期胚胎冷冻保存时间不同将941例胚胎冻融周期分为3组：1~〈9个月组（749例）、9~〈16个月组（144例）与≥16个月组（48例）。比较3组的胚胎存在率、复苏存活率、完整胚胎存活率、基本完整胚胎率、胚胎种植率、生化妊娠率、临床妊娠率、异位妊娠率。结果 3组胚胎复苏存活率（分别为98.22%、98.06%和96.33%）、完整胚胎存活率（分别为86.95%、82.58%和88.07%）、基本完整胚胎率（分别为10.78%、14.19%和7.33%）、生化妊娠率（分别为62.22%、54.17%和54.17%）、临床妊娠率（分别为58.74%、49.31%和52.08%）、胚胎种植率（分别为40.53%、33.22%和37.23%）相比较,差异均无统计学意义（P〉0.05）。结论 在所研究的胚胎冷冻期限内,胚胎玻璃化冷冻保存时间不影响解冻后胚胎质量与妊娠结局。
Objective： To evaluate the influence of cryopreservation duration on quality of frozen-thawed Day 3 embryo and pregnancy outcome in frozen-thawed embryo transfer（FET）cyeles. Methods： In this retrospective study, 941 FET cycles were divided into 3 groups according to the storage duration of vitrified embryos ： （ 1-〈9 ） months （ 749 cycles）, （ 9-〈 16 ） months （ 144 cycles）,≥ 16 month（48 cycles）. The rates of embryos survival,fully intact blastomere,substantially intact blastomere, biochemical pregnancy,clinical pregnancy and implantation were evaluated. Results： Among the three groups,the survival rates were 98.22% ,98.06% and 96. 33% ;fully intact blastomere rates 86.95% ,82.58% and 88.07% ;substantially intact blastomere rates 10.78% ,14.19% and 7.33%;biochemical pregnancy rate 62.22%, 54.17% and 54.17%; clinical pregnant rates 58.74%, 49.31% and 52.08%; implantation rates 40.53%,33.22% and 37.23%, respectively. There were no significant differences in all observed parameters among the three groups（P〉0.05）. Conclusions： Within the studied cryopreservation duration of vitrified embryo, the storage duration does not affect the quality of frozen-thawed embryo and the pregnancy outcomes of FET cycles.
出处
《生殖医学杂志》
CAS
2015年第11期906-910,共5页
Journal of Reproductive Medicine
基金
生殖遗传教育部重点实验室(浙江大学)/浙江省女性生殖健康重点实验室开放基金资助项目资助(2012-RG/RH-0003)
关键词
冷冻保存时间
玻璃化
胚胎质量
妊娠结局
Cryopreservation duration
Vitrification
Embryo quality
Pregnancy outcome
