摘要
目的研究miR-16在慢性不可预知温和应激抑郁症模型大鼠神经系统不同部位的表达及其与5-羟色胺(5-HT)转运体的关联性。方法对照组和慢性不可预知温和应激抑郁模型组大鼠各10只,模型组大鼠接受21天的不可预知温和应激刺激,对照组大鼠正常饲养。两组大鼠麻醉后收集脑脊液,测定miR-16。然后断头处死,分离前额叶皮质、中缝核、海马,测定miR-16和5-HT转运体蛋白。结果模型组大鼠脑脊液和中缝核的miR-16相对表达量低于对照组,中缝核miR-16与5-HT胺转运体呈负相关;而前额叶皮质和海马中的miR-16、5-HT转运体分别与对照组比较,差异均无统计学意义(P>0.05)。结论中缝核miR-16可能通过调节5-HT转运体的表达,参与抑郁症的病理过程,脑脊液miR-16亦可能与抑郁症相关。
Objective To explore the expression of miR -16 and its association with serotonin transporter in multi -tissues from the rat model of chronic unpredictable mild stress -induced depression .Methods SD rats were randomly divided into the control group and the depression group.Rats in the depression group experienced unpredictable mild stressors for 3 weeks, while rats in the control group re-ceived no treatment .MiR-16 in cerebrospinal fluid , prefrontal cortex , hippocampus , and raphe was detected by real -time PCR.Sero-tonin transporter protein in prefrontal cortex , hippocampus , and raphe was detected by Western blotting .Results The relative expression levels of miR-16 in cerebrospinal fluid and raphe of the depression group were significantly lower than those of the control group .In ra-phe, the miR -16 level was negatively associated with expression of serotonin transporter protein .However, there was no significant difference of miR-16 expression in prefrontal cortex and hippocampus between the depression group and the control group .Conclusion MiR-16 in raphe may involve in the pathological process of depression via regulation of serotonin transporter expression .MiR-16 in ce-rebrospinal fluid may also associate with depression .
出处
《医学研究杂志》
2015年第10期42-46,共5页
Journal of Medical Research
基金
浙江省自然科学基金资助项目(LQ13H090003)
杭州市科技发展计划项目重点专科专病科研攻关专项基金资助项目(20130733Q26
20130733Q27)
