摘要
从山东保护地土壤中分离得到木霉菌Y15,结合ITS、tef1-α和形态学特征进行鉴定.结果发现Y15菌株为近深绿木霉（Trichoderma paratroviride）.该菌株在PDA上菌落稠密,老熟后毡状,灰绿色;分生孢子梗主轴长,着生短的二次分枝,再次分枝少,分枝成对或轮生.瓶梗通常2～4个轮状排列,烧瓶形或锥形,直或弯曲;分生孢子亚球形至卵圆形,绿色,光滑.tef1-α序列与近深绿木霉模式菌株S489和S385序列同源性高达99％,在系统进化树中与其亲缘关系也最近.该种在国内首次报道和描述,为木霉属中国新记录种.
Trichoderma strain Y15 was isolated from the vegetable soil under Shandong greenhouse, and identified by analysis of internal transcribed spacer regions of the rRNA gene cluster （ITS）, partial sequences of transcription extensions factor 1-alpha （tell-co） and morphological characteristics. Results show that Y15 is T. paratroviride. The strain is colony dense, panniform, greyish-green on PDA. Conidiophore main axes is long with short secondary branches, not extensively rebranching. Its branches are coupled or verticillate. Phialides usually has 2 ~ 4 verticils, lageniform or subulate, straight or flexuous. Conidia is subglobose to obovoid, greenish and smooth. The tef1-α sequence of strain Y15 has 99% homology with strains S489 and S385 of T. paratroviride type, and has the closest genetic relationship with them in phylogenetic tree. It is originally reported in China as a new Chinese record of genus Trichoderma.
出处
《山东科学》
CAS
2015年第5期35-40,共6页
Shandong Science
基金
山东省科技发展计划(2014GSF121028
2013GNC20119)
国家自然科学基金(31572044)
国家科技基础性工作专项(2014FY120900)
