摘要
目的:观察右美托咪啶对脓毒症大鼠肾脏功能及Toll受体4表达的影响,探讨减轻脓毒症损害的潜在治疗方案。方法:选择雄性SD大鼠作为研究对象,采取盲肠结扎穿孔方法建立脓毒症模型;取模型制备成功的大鼠按照数字随机法分为3组,A组为空白对照组,泵注1.0 m L/kg生理盐水,B组为对照组,术毕泵注1.0 m L/kg生理盐水,维持10 min,持续泵注NS 1.0 m L/kg·h,C组为实验组,术毕给予右美托咪定5μg/kg,维持10 min,持续泵注右美托咪定4.5μg/kg·h,3组均持续泵注24 h,并于6 h、12 h、24 h各组分别取8只大鼠检测中性粒细胞明胶酶相关脂质运载蛋白(NGAL)表达水平进行比较,并观察比较3组研究对象尿素氮(BUN)、肌酐清除率(Ccr)以及肾脏组织Toll受体4水平及TLR4 m RNA水平。结果:C组大鼠应用右美托咪定后,BUN于6 h、12 h、24 h分别为(13.4±1.2)mmol/L、(12.5±1.3)mmol/L、(11.4±1.1)mmol/L,均低于A组、B组,均P<0.05;Ccr于6 h、12 h、24 h分别为(77.4±9.9)m L/min、(75.4±10.2)m L/min、(78.5±11.3)m L/min,均明显低于A组、B组,P<0.05;C组6 h、12 h、24 h NGAL表达水平分别为(39.2±9.9)pg/m L、(41.6±9.8)pg/m L、(38.2±9.9)pg/m L,均明显低于A组、B组,P<0.05;C组泵注6 h、12 h、24 h Toll受体4水平及TLR4 m RNA水平均低于A组、B组,P<0.05。结论:右美托咪啶可降低脓毒症大鼠Toll受体4表达,有助于减轻脓毒症炎性反应与减轻肾损害,具有重要临床价值。
Objective： To observe the effect of dexmedetomidine on renal function and the expression of Toll receptor 4 of sepsis rat models, to investigate the potential therapeutic scheme to reduce damage induced by sepsis. Methods： SD rats were selected as the research objects, and they were divided into 3 groups according to random number table. All three groups were performed cecal ligation and puncture to establish the model of sepsis. The rats were injected 5 mg/kg LPS through tail vein indwelling needle; after establishing the model, A group was blank control group which was treated with 1.0 m L/kg normal saline infusion, and the rats of B group was treated with 1.0 m L/kg normal saline infusion with maintaining 10 min, then continuous NS 1.0 m L/kg·h, and the rats of C group received 5μg/kg dexmedetomidine with maintaining 10 min, then continuous infusion of dextromethorphan with 4.5 μg/kg·h. All groups maintain continuous infusion for 24 h. The expression level of neutrophils enzyme related lipid carrier protein gelatin（NGAL） in 6 h, 12 h, 24 h was detected and the level of blood urea nitrogen（BUN） and creatinine clearance rate（Ccr） and Toll receptors 4 and TLR4 m RNA was detected and compared among groups. Results： The BUN of group C after the application of dexmedetomidine on 6 h, 12 h, 24 h were（13.4 ± 1.2） mmol/L,（12.5 ± 1.3） mmol/L,（11.4 ± 1.1） mmol/L and were lower than those of group A, group B, P〈0.05; the Ccr of group C on 6 h, 12 h and 24 h were（77.4 ± 9.9） m L/min,（75.4 ± 10.2） m L/min,（78.5 ± 11.3） m L/min and were significantly lower than those of group A, group B, P〈0.05; the level of NGAL expression of group C on 6 h,12 h, 24 h were（39.2 ± 9.9） pg/m L,（41.6 ±9.8） pg/m L,（38.2 ± 9.9） pg/m L, obviously lower than that of group A and B, P〈0.05. The level of TLR4 and TLR4 m RNA of group C on 6 h, 12 h, 24 h were lower than those of group A and B, P〈0.05. Conclusions： Dexmedetomidine can reduce the expression of Toll receptor 4 i
出处
《现代生物医学进展》
CAS
2015年第28期5435-5438,5499,共5页
Progress in Modern Biomedicine
基金
国家自然科学基金项目(81141065)
