摘要
目的：探讨银杏内酯N抗谷氨酸诱导大鼠PC12细胞损伤的影响及机制。方法：使用谷氨酸建立PC12细胞损伤模型,给予不同浓度银杏内酯N,并以银杏内酯B为阳性对照。采用MTT比色法测定细胞存活率;采用吖啶橙（AO）染色检测银杏内酯N对PC12凋亡的作用;采用流式细胞术检测银杏内酯N对PC12细胞活性氧（ROS）、线粒体膜电位的影响,采用Western blot检测Cleaved Caspase-3蛋白的表达。结果：银杏内酯N在2～8μmol/L剂量时,可抑制谷氨酸诱导的PC12细胞凋亡及ROS的升高,稳定受损PC12细胞的膜电位,降低Cleaved Caspase-3蛋白表达。结论：银杏内酯N能拮抗谷氨酸导致的PC12细胞损伤,其机理与减少ROS的生成,稳定细胞膜电位,抑制Cleaved Caspase-3蛋白表达有关。
Objective：To study the protective effects of ginkgolide N against glutamte-induced injury in PC12 cells and its mecha- nisms. Methods：The injury model was established by treating PC12 cells with glutamate, and PC12 cells were treated with different con- centrations of ginkgolide N with ginkgolide B as control. The cells activity was analyzed by MTT assay. The apoptosis of PC12 cells were examined by acridine orange （AO）staining, the reactive oxygen species and mitochondrial membrane potential of PC12 cells were exam- ined by flow cytometry. Western blot method was used to examine the expression of Cleaved Caspase-3 protein. Results ： Ginkgolides N of 2 -8 μmol/L inhibited PC12 cells apoptosis and ROS accumulation induced by glutamate, stabilized membrane potential of damaged PC12,and reduced the expression of Cleaved Caspase-3 protein. Conclusion： Ginkgolide N has a protective effect on PC12 ceils injury induced by glutamate, and the mechanism may be associated with reducing ROS generation, stabilizing membrane potential and inhibi- ting the expression of Cleaved Caspase-3 protein.
出处
《中药材》
CAS
CSCD
北大核心
2015年第8期1694-1698,共5页
Journal of Chinese Medicinal Materials
基金
广东省中医药局项目(20121106)
广东食品药品职业学院院级项目(2011YZ001)
