摘要
目的探讨白细胞介素-8(interleukin-8,IL-8)水平在猪脑死亡模型的变化及其与肾功能指标的相关性。方法采用标准实验长白小猪7头,手术准备操作后取脑死亡前静脉血,硬膜外颅内生理盐水加压至脑死亡,分别在确立脑死亡时间点及脑死亡后3 h、6 h、9 h取静脉血。以免疫芯片荧光定量法检测脑死亡前、脑死亡0 h、脑死亡后3 h、6 h、9 h血清IL-8浓度,以生物化学法检测尿素、肌酐(creatinine,Cr)水平,对检测结果进行统计学分析。结果脑死亡后IL-8检测结果开始升高,3 h、6 h、9h的IL-18检测结果均高于脑死亡前,且差异均有统计学意义(P均<0.05)。脑死亡后各时间点IL-8的检测结果差异无统计学意义(H=7.840,P=0.098)。脑死亡后3 h、6 h、9 h的尿素检测结果均高于脑死亡前,差异均有统计学意义(P均<0.05)。脑死亡后6 h、9 h的Cr检测结果均高于脑死亡前,差异均有统计学意义(P均<0.05)。脑死亡前后血清IL-8与尿素检测结果呈正相关性(r=0.453,P=0.012),与Cr检测结果无相关性(r=0.209,P=0.267)。结论脑死亡可引起组织释放IL-8,并可造成组织细胞及肾功能不可逆性损伤。因此,临床为保护潜在移植器官应在脑死亡确立以后及时采取有效的血液净化吸附IL-8,以降低组织器官的炎性损伤。
Objective To study the change of interleukin-8（IL-8） and its correlation with renal function index in brain dead organ donor model. Methods 7 heads of standard experimental Changbai pigs were used. Blood samples were taken after operation preparation and at immediately time as well as 3 h, 6 h, 9h after brain death which caused by method of epidural intracranial pressure. Serum IL-8 concentration was determined by immune chip fluorescent quantitative method and the corresponding kidney function indexes were detected by biochemistry methods. The results were analyzed statistically. Results Serum IL-8 concentration increased gradually after brain death. The concentration of IL-8 at 3 h, 6 h, 9 h after brain death were all higher than that of before brain death, and the differences all had statistical significance（Pall 〈0.05）.There was no statistical significance in the differences of IL-8 concentration among different time after brain death（H= 7.840, P= 0.098）. The urea levels at 3 h, 6 h, 9 h after brain death were all higher than that of before brain death, and the differences all had statistical significance（Pall 〈0.05）. The creatinine（Cr） levels at6 h, 9h after brain death were all higher than that of before brain death, and the differences all had statistical significance（Pall 〈0.05）. The concentration of IL-8 was positively correlated with urea（r= 0.453, P= 0.012）,but not correlated with Cr（r= 0.209, P= 0.267）. Conclusion Brain death induce plenty of IL-8 released,and which can cause irreversible damage to the tissue cells and renal function. Therefore, the clinical measures should be taken as soon as possible after brain death in order to reduce the organ inflammatory damage of donation after brain death.
出处
《实用检验医师杂志》
2015年第3期169-172,共4页
Chinese Journal of Clinical Pathologist
基金
国家临床重点专科建设项目[国卫医函(2013)544号]
关键词
脑死亡
白细胞介素-8
炎性反应
肾功能
Brain dead
Interleukin-8
Inflammatory response
Renal function
