摘要
目的探讨恶性黑素瘤组织中iNOS表达与增生及临床资料相关性。方法收集30例恶性黑素瘤患者皮损组织和20例色素痣患者的皮损组织,采用免疫组化检测恶性黑素瘤和色素痣组织中iNOS的表达,并与患者临床资料进行分析,同时观察其与癌细胞增生、血管生成的关系。结果 iNOS表达阳性率在恶性黑素瘤组织中比色素痣组织高（P〈0.05）,恶性黑素瘤iNOS表达在患者年龄、性别、部位中差异无统计学意义（P〉0.05）。病理分期Ⅲ~Ⅳ期患者iNOS阳性率显著高于Ⅰ~Ⅱ期（P〈0.05）。iNOS阳性肿瘤患者细胞增生核抗原（PCNA）标志指数（PLI）和微血管密度（MVD）的值明显高于iNOS阴性肿瘤患者（P〈0.01）。结论 iNOS能促进恶性黑素瘤细胞增生与血管形成,是恶性黑素瘤的重要调节因子。
Objective To investigate the expression of iNOS in malignant melanoma（MM） and its correlation with the cancer cell proliferation,angiogenesis and clinical data,as well as its effects on the development of MM. Methods Total 30 cases of MM tissues and 20 cases of nevus were collected,and the immunohistochemical method was adopted to detect the expression of iNOS,then the clinical data were analyzed,finally the relationship between the cancer cell proliferation and angiogenesis was observed. Results The positive rate of iNOS in MM was higer than that of nevus group（P〈0.05）. In MM group,there was no relevance between the expression of iNOS and the gender,location and tumor growth pattern（P〉0.05）. The iNOS positive rate of pathological staging III-IV was significantly higher than that of I-II（P〈0.05）. The PLI and MVD value of iNOS positive tumors was obviously higher than that of iNOS negative tumors（P〈0.01）. Conclusion The expression of iNOS can promote the proliferation of cancer cells and vessel formation in MM,which is an important regulatory factor in tumor biological behavior.
出处
《实用皮肤病学杂志》
2015年第5期329-331,共3页
Journal of Practical Dermatology
基金
湖南省自然科学基金青年基金项目(09JJ4011)
湖南省人民医院仁术科研基金项目
关键词
黑素瘤
恶性
INOS
细胞增生核抗原
血管生成
Melanoma
malignant
iNOS
Proliferating cell nuclear antigen
Angiogenesis
