摘要
目的 应用非显带技术,分析人体肝癌细胞系SMMC-7721的染色体核型.方法 体外培养SMMC-7721细胞,通过秋水仙素使细胞同步化,低渗、滴片、吉姆萨染色,进行非显带染色体核型分析.结果 数目方面,SMMC-7721细胞系核型的众数为60-70条,峰值主要出现在E组,通过分析染色体数目在50-70个之间的较清晰的25个核型发现,E、F、G组染色体总数大多过半甚至超过60%;结构方面,分辨出双着丝粒染色体、含有3个着丝粒的染色体和环状染色体.结论 上述染色体的数目异常与结构畸变,对认识肝细胞的转化、恶变以及临床的初步诊治提供了细胞遗传学基础.
Objective To analyze karyotype of human hepatoma cell lines SMMC-7721 using non-banding technique. Methods SMMC-7721 cells were cultured in vitro, followed by cell synchronization with colchicine, hy-potonic treatment and Giemsa staining, and then non-banding karyotype was analyzed. Results The majority number of karyotypes of SMMC-7721 cell lines was 60 to 70. Peaks occurred mainly in the E group, and the majority of E, F, G groups were more than half of the total number of chromosomes or even more than 60%（25/128）. For the structure of chromosomes, dicentric chromosome, chromosome sharing three centromeres and ring chromosome could be found in a few cells. Conclusion The analysis of the abnormal chromosome will help us to deeply understand transforma-tion and deterioration of liver cells, which provides the genetic basis for preliminary clinical diagnosis and treatment.
出处
《海南医学》
CAS
2015年第20期2967-2969,共3页
Hainan Medical Journal
基金
西安医学院校级重点学科细胞生物学
西安医学院校级精品资源共享课程(编号:XYZL2014-3)
陕西省大学生创新创业计划(编号:201211840010
201211840011)
陕西省优势学科建设经费资助
