摘要
背景:骨髓间充质干细胞可以在体内外诱导下分泌神经营养因子,还可向神经元方向转化,从而促进创伤脑组织再生修复,但骨髓间充质干细胞又存在生存周期短的问题而影响其发挥对损伤脑组织的保护作用。目的:观察骨髓间充质干细胞移植联合神经节苷脂治疗大鼠颅脑创伤的效果。方法:60只Wistar大鼠采用液压颅脑损伤仪制备大鼠重型颅脑创伤模型,建模24 h后按照随机表法随机分为3组:模型组经尾静脉注入1 m L DMEM培养液,移植组经尾静脉注入1 m L(1×1010 L-1)骨髓间充质干细胞悬液,联合组经尾静脉注入1 m L(1×1010 L-1)骨髓间充质干细胞悬液以及经腹腔注射神经节苷脂水溶液(30 mg/kg),1次/d,共3 d。伤后24 h,细胞移植后3 d及1,2,3,4周依据Longa 5分法进行神经功能行为学评分;移植后3 d应用RT-PCR、Western blot检测创伤脑组织中水通道蛋白4基因和蛋白的表达;移植后1周,行苏木精-伊红染色观察创伤脑组织病理变化。结果与结论:移植后3 d及1,2,3,4周,联合组神经行为学功能评分低于移植组(P<0.05),移植组低于模型组(P<0.05);移植后3 d,模型组创伤脑周围组织水通道蛋白4蛋白及m RNA的表达高于移植组(P<0.05),移植组高于联合组(P<0.05);苏木精-伊红染色观察到联合组脑组织恢复明显优于模型组和移植组(P<0.05)。结果提示骨髓间充质干细胞移植联合神经节苷脂可以明显改善颅脑创伤大鼠的神经行为学功能。
BACKGROUND： Bone marrow mesenchymal stem cells can secrete neurotrophic factors in vitro, and can also be differentiated into neurons, thereby contributing to the repair of traumatic brain injury. However, the short life cycle of bone marrow mesenchymal stem cells influences their protective effects on the damaged brain tissues. OBJECTIVE： To observe the effect of bone marrow mesenchymal stem cell transplantation combined with ganglioside in rats with traumatic brain injury. METHODS： Sixty Wistar rats were used to make severe traumatic brain injury models using a hydraulic head injury instrument, and then randomized into three groups： 1 mL DMEM, 1 mL bone marrow mesenchymal stem cell suspension（1×10^10/L）, 1 mL bone marrow mesenchymal stem cell suspension（1×10^10/L） combined with ganglioside solution（30 mg/kg） were injected respectively in model group, transplantation group and combined group, once a day, totally for 3 days. Neurological behavior scores were observed according to Longa method at 24 hours after modeling and at 3 days, 1, 2, 3, 4 weeks after cell transplantation. At 3 days after cell transplantation, RT-PCR and western blot assay were employed to detect aquaporin 4 m RNA and protein expressions. At 1 week after transplantation, hematoxylin-eosin staining was performed for pathological observation of the damaged brain tissues. RESULTS AND CONCLUSION： At 3 days, 1, 2, 3, 4 weeks after cell transplantation, the neurological behavior scores were ranked as follows： combined group 〈transplantation group 〈model group（P〈0.05）. At 3 days aftercell transplantation, the m RNA and protein expression of aquaporin 4 was ranked as follows： model group〉 transplantation group〉 combined group（P〈0.05）. Hematoxylin-eosin staining showed that the recovery of brain tissue was better in the combined group than the model and transplantation groups（P〈0.05）. These findings indicate that bone marrow mesenchymal stem cells combined with ganglioside can significantly improv
出处
《中国组织工程研究》
CAS
北大核心
2015年第36期5811-5815,共5页
Chinese Journal of Tissue Engineering Research
关键词
干细胞
移植
颅脑创伤
神经节苷脂
骨髓间充质干细胞
大鼠
Cerebral Hemorrhage
Traumatic
Gangliosides
Bone Marrow
Mesenchymal Stem Cell Transplantation
