摘要
中缅树鼩是分布于热带地区的物种。为研究在寒冷环境下中缅树鼩下丘脑神经肽对其生理适应性调节作用,本研究测定了经冷驯化处理后中缅树鼩的体重、摄食量、血清瘦素浓度以及下丘脑多种神经肽表达量的变化。结果显示:在冷驯化条件下,中缅树鼩体重和摄食量增加,血清瘦素浓度显著下降;神经肽Y(Neuropeptide Y,NPY)表达量上升,可卡因—苯丙胺调节转录肽(Cocaine and amphetamine regulated transcript peptide,CART)和阿片促黑色素原(Pro-opiomelanocortin,POMC)表达量降低,豚鼠相关蛋白(Agouti related peptide,AgRp)表达量无显著性差异。表明在冷驯化条件下,中缅树鼩主要通过改变NPY、CART和POMC基因的表达水平等参与调节能量代谢。
Tree shrew（Tupaia belangeri） is a species which is distributed in tropical and subtropical regions. In the present study,we tested the hypothesis that hypothalamic neuropetides were cues is to induce adjustments in body mass regulation and metabolism in cold-exposed Tupaia belangeri. Body mass,food intake and thermogenic capacity,serum leptin levels and hypothalamic neuropeptide expression levels were measured. The animals were subjected to either 5℃ for 28 days in cold-exposed,or maintained under 25℃ for 28 days. The results showed a significant increase in body mass and food intake in the cold exposure group,accompanied by a significant decrease in serum leptin levels,hypothalanic cocaine and amphetamine regulated transcript peptide（CART） mRNA and pro-opiomelanocortin（POMC） mRNA levels. Neuropeptide Y（NPY） mRNA was significantly increased in the cold exposure group,but there were no changes in hypothalamic agoutirelated protein（AgRP） mRNA levels between the control and cold acclimation groups. All of the results suggest that hypoleptinemia partially contributes to cold-induced hyperphagia,which might involve the elevation of hypothalamic NPY,CART and POMC gene expression.
出处
《兽类学报》
CAS
CSCD
北大核心
2015年第4期438-444,共7页
Acta Theriologica Sinica
基金
国家国际科技合作专项项目(2014DFR31040)
十二五科技支撑项目(2014BAI01B00)
国家自然科学基金项目(31360096)
关键词
下丘脑神经肽
冷驯化
中缅树鼩
能量调节
Cold acclimation
Energy regulation
Hypothalamic neuropatide
Tupaia belangeri
