摘要
目的研究胃癌组织及其邻近胃组织中睾丸特异性Y样蛋白5(testis-specific Y-like protein 5,TSPYL5)的表达与预后及多个临床病理变量之间的关系,同时分析其是否在胃癌远期生存的危险因素中具有意义。方法采用RT-PCR方法检测44例胃癌组织、癌旁胃正常黏膜组织中TSPYL5 mRNA的表达,分析其表达与胃癌相关临床病理变量的关系。结果与癌旁组织相比,胃癌组织中TSPYL5表达明显下调的有24例,占总数的54.5%。TSPYL5的表达与TNM分期、淋巴转移及是否远处转移有关,而与肿瘤的浸润深度、大小及患者性别、年龄等无关(P<0.05)。肿瘤组织与癌旁组织的TSPYL5表达量相比,其表达下调者的DFS远低于无明显变化及上调者(P=0.01)。经单因素和多因素COX分析表明,TSPYL5可作为胃癌的独立预测因素(P<0.001)。结论 TSPYL5作为抑癌基因,其表达缺失参与了胃癌的发生发展,同时TSPYL5可作为判断胃癌预后的重要分子标志。
Objective To study the relationship between the expression of TSPYL5 （ testis-specific Y-like protein 5,TSPYL5）in gastric carcinoma and adjacent gastric tissues and the long term survival. Our study is to investigate the clinical significances of expression of TSPYL5 in gastric cancer. Methods The mRNA level of TSPYL5 in 44 matched samples comprising primary gastric carcinoma and paired non-cancerous mucosa were detected and compared by quantitative RT-PCR. Then the correlations of mRNA level of TSPYL5 with the clini-copathological characteristics and prognosis of patients with gastric cancer were analyzed. Results The rate of TSPYL5 mRNA in the primary gastric cancer tissues was significantly higher than that in the adjacent non-can-cerous tissues（54. 5%）. mRNA expression of TSPYL5 was associated with certain clinical-pathologic variables such as advancing TNM stage, lymphatic metastasis, distant metastasis. We obtained the expression levels ratio of cancer tissue and adjacent non-cancerous tissues and found the lower ratio with a lower Disease-Free Survival （DFS）（P〈0. 001）. TSPYL5,as a tumor suppressor gene, remained a statistically-significant prognostic marker＆amp;nbsp;in the COX regression analysis. Conclusion Down-expression of TSPYL5 had a significant effect on develop-ment and progress in gastric cancer,which can be used to prognose gastric carcinoma.
出处
《中国肿瘤外科杂志》
CAS
2015年第5期278-281,298,共5页
Chinese Journal of Surgical Oncology
基金
六大人才高峰(2013-WSW-028)
关键词
TSPYL5
抑癌基因
甲基化
胃癌
预后
TSPYL5
tumor suppressor gene
methylation
gastric cancer
prognostic
