摘要
目的 探讨血管紧张素原（AGT）T704C rs699位点多态性与小动脉粥样硬化性脑梗死（SA）发病机制的关系.方法 以SA组186例和对照组94例为研究对象,采用SNaPshot技术及GeneMapper4.0软件分析AGT T704C rs699位点的基因分型情况,比较C（T）基因特征及等位基因频率,并进一步采用多元logistic回归,分析脑血管病常见危险因素与所携带基因分型在SA发病中的交互作用.结果 AGT T704C rs699位点在SA组中以携带TT和TC基因型多见（30.1％、41.9％）,但患病率以CC基因型为高（80.0％）,与TT和TC基因型（65.1％、60.4％）比较,差异均有统计学意义（χ2=5.24、6.78,均P＜0.05）;SA组T等位基因频率高于C等位基因频率,但差异无统计学意义（χ2=1.23,P ＞0.05）.携带AGT CC基因型（OR=3.34,95％CI 1.54 ～ 8.43）、高血压病史（OR=5.59,95％CI 2.35 ～ 22.61）、老龄（≥70岁）（0R=2.82,95％CI 1.13～ 10.63）是SA发病的独立危险因素. 结论 AGT T704C基因多态性可能是SA发病机制的遗传因素,且携带AGT CC基因型,高血压病史和老年患者具有协同致病效应.
Objective To investigate the association of small vessel atherosclerotic cerebral infarction （SA）. Methods angiotensinogen gene （AGT） T704C rs699 polymorphism with One hundred and eighty six patients with small atherosclerotic cerebral infarction and 94 healthy individuals（control group） were enrolled in the study. AGT T704C （rs699） genotype was detect- ed with SNaPshot technique and analyzed with GeneMapper4.0 software. The genotypes of AGT T704C and allele frequency were compared between the two groups, and the risk factors of SA was analyzed with multi-dimensional Logistic regression. Results The TT and TC genotypes were 30.1%, 41.9% in rs699 sites of SA patients. The prevalence of SA was higher in subjects with CC genotype （80.0%）, which was significantly different to those with TT and TC genotypes （65.1% and 60.4%, χ2=5.24 and 6.78, all P〈0.05）. There was no significant difference in frequency of T and C alleles between SA and control groups （χ2=1. 23, P 〉0.05）. Multivariate Logistic regression analysis showed that AGT CC genotype（OR=3.34, 95%CI：1.54-8.43）, hypertension （OR=5.59, 95% CI： 2.35~22.61）, age（≥70 y） （0R=2.82, 95% CI：1.13- 10.63） were the independent risk factors for SA. Conclusion The polymorphism of AGT T704C may be associated with SA, AGT CC genotype, hypertension and old age are risk factors of SA.
出处
《浙江医学》
CAS
2015年第17期1436-1439,共4页
Zhejiang Medical Journal
基金
湖州市科技局一般项目(2013GYB05)
