摘要
目的研究生脉、地塞米松联合局部低温对大鼠睾丸扭转复位后生精细胞凋亡的影响。方法将48只青春期标准SD大鼠随机分为6组(A、B、C、D、E、F组),每组8只。6组大鼠分别扭转左侧睾丸720°持续2 h,建立单侧睾丸扭转模型。A组:常温+生理盐水;B组:常温+生脉+地塞米松;C组:低温+生理盐水;D组:低温+地塞米松;E组:低温+生脉;F组:低温+生脉+地塞米松。术后48 h采集睾丸,通过HE染色光镜观察睾丸组织病理学改变,用原位缺口末端标记法(TUNEL)法检测睾丸生精细胞的凋亡。结果 B、C、D、E组,睾丸组织可见部分生精细胞脱落;F组,小鼠睾丸内出现生精细胞脱落现象。凋亡指数(AI),A组(35.13±2.21)明显高于B组(18.12±5.23)、C组(17.13±3.03)、D组(17.05±1.03)、E组(16.39±2.02;均P<0.05)及F组(9.06±1.53;P<0.01)。结论睾丸扭转复位后缺血再灌注损伤可导致生精细胞凋亡增加;局部低温联合生脉、地塞米松对睾丸扭转复位后生精细胞凋亡具有良好保护作用。
Objective To investigate the protective effect of hypothermia union Shengmai and dexamethasone on spermatogenie cell apoptosis of torsioned testes in rats. Methods Forty- eight adolescence male Spra- gue -Dawley rats were random divided into 6 groups. Each group of 8 rats, six group rats were left testieular torsion 720° for 2 h, establish a u- nilateral testicular torsion model. Group A ： normal temperature ＋ saline ; group B ： normal ＋ Shengmai ＋ dexamethasone ; group C ~ low temperature ＋ saline ; group D ： low temperature ＋ dexamethasone ; group E ： low tem- perature ＋ Shengmai ; group F： Shengmai ＋ dexamethasone ＋ low tempera- ture. The testicles were collected after 48 h, the testicular histopathologi- cal changes were observed by HE staining light microscopy. The apopto- tic spermatogenic cells law were detected by TdT -mediated dUTP nick -end labeling （TUNEL） . Results Group B, C, D and E testicular tissue pathology damage visible parts of pure cell falls off born. The born small tube of testicular sperm cells in group F is born slightly off, general structure and tidy. Apoptosis index （AI） in group A （ 35.13 ± 2. 21 ） was higher than that group B （ 18. 12 ± 5.23 ）, group C （ 17.13 ±3.03 ）, groupD （17.05±1.03）, group E （16.39±2.02;allP〈0.05） and group F （ 9.06 ± 1.53 ; P 〈 0.01 ） . Conclusion Testicular torsion reduction ischemia- reperfusion injury can lead to life essence cell apoptosis increase. Hypothermia union Shengmai and dexa-methasone on spermatogenic cell apoptosis of torsioned testes in rats have good protection.
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2015年第20期2037-2039,共3页
The Chinese Journal of Clinical Pharmacology
基金
福建省宁德市科技计划指导基金资助项目(20110094)
关键词
睾丸扭转
低温
生脉
地塞米松
生精细胞凋亡
testicular torsion
hypothermia
Shengmai
dexamethasone
spermatogenic cell apoptosis
