摘要
目的对2012年陕西省连续发生的两起B型流感暴发疫情进行实验室诊断,分析暴发疫情分离株的HA基因、NA基因的基因特征和变异特点。方法对两起暴发疫情流感样病例标本进行real-time PCR核酸检测,用MDCK细胞培养流感病毒,提取病毒核酸,用RT-PCR扩增病毒HA基因和NA基因,测定核苷酸序列,用DNAman、Mega软件分析结果。结果两起流感暴发疫情均由B型流感病毒Victoria系引起。其HA基因与2010年-2011年北半球疫苗株相比,无核苷酸的丢失、插入、糖基化位点的改变,氨基酸的同源性分别为99.15%、98.97%。其NA基因与2010年-2011年北半球疫苗株相比,无核苷酸的丢失、插入、糖基化位点的改变,氨基酸的同源性均为96.35%。两起暴发疫情之间的HA氨基酸序列、NA氨基酸序列的同源性分别为99.83%、99.79%。结论引起两起暴发疫情的流感病毒为同一流行株,未发现耐药株,对神经氨酸酶抑制剂依然敏感。
Objective To diagnose consecutive occurrence of two type B influenza outbreaks in Shaanxi in 2012,to analyze HA and NA genes characteristics and their mutation of influenza B virus which was isolated from influenza outbreaks. Methods Real- time PCR was used to detect influenza- like cases specimens of the two influenza outbreaks,the influenza virus were cultured in MDCK cells. RNAs were extracted and HA,NA gene were amplified by RT- PCR,the PCR products were sequenced and analyzed with DNAman and Mega software. Results The two outbreaks were caused by Victoria lineages influenza B virus. HA compared with vaccine strain during 2010- 2011 of the northern hemisphere,there was no deletion or insertion or change of glycosylation site,and the homology of amino acids was respectively 99. 15 %,98. 97%. NA compared with vaccine strain during 2010-2011 of the northern hemisphere,there was no deletion or insertion or change of glycosylation site,and the homology of amino acids was 96. 35%. The homology of the two outbreaks in the HA amino acid sequence and NA amino acid sequence was99. 83%,99. 79%,respectively. Conclusion The influenza virus which caused two outbreaks were the same epidemic strain,resistant strains were not found,which were all sensitive to neuraminidase- inhibitor drugs.
出处
《中国卫生检验杂志》
CAS
2015年第19期3349-3352,共4页
Chinese Journal of Health Laboratory Technology
基金
"十二五"国家科技重大专项(2013ZX10004202-001-002)
陕西省卫生厅科研基金核定经费项目(08H11)
关键词
流感
暴发
快速诊断
分子流行病学调查
Influenza
Outbreak
Rapid diagnosis
Molecular epidemiology investigation
