摘要
目的探讨原发性乳腺癌患者外周血循环肿瘤细胞和肿瘤干细胞的组织病理学相关指标及其与预后的相关性,以期为原发性乳腺癌临床诊断和预后判断提供科学依据。方法选取2011年1月至2014年1月间收治的原发性乳腺癌患者42例,制备外周血单个核细胞混悬液（PBMC）,同时选取同期健康体检的健康者20例作为对照,PBMC标记的细胞角蛋白（CK）19单克隆抗体,并作为外周血循环肿瘤细胞标记物进行CK19阳性细胞检测;免疫荧光显微镜对CD44＋/CD24-/low的循环肿瘤细胞（CTC）含量进行检测,分析和比较两组受试者CK19阳性细胞。同时将观察组患者按照临床分期和淋巴转移情况进行分组,分析亚组患者的CK19阳性细胞检测情况;对CTC阳性率肿瘤干细胞（TSC）阳性率以及其与临床分期等相关性进行分析。结果观察组患者CK19阳性率为76.2%,健康对照组无阳性细胞,差异有统计学意义（P〈0.05）;淋巴结转移情况为N0的患者CK19阳性率（59.1%）明显低于淋巴结转移情况为N1~3的患者（95.0%）,差异有统计学意义（P〈0.05）;临床Ⅰ期患者CK19的阳性率53.8%,明显低于Ⅱ期（84.2%）和Ⅲ期（90.0%）,差异均有统计学意义（均P〈0.05）;淋巴结转移情况为N0的患者CD44＋/CD24-/low阳性率（46.2%）低于淋巴结转移情况为N1~3的患者（78.9%）,但差异无统计学意义（P〉0.05）;而临床Ⅰ期CK19阳性率（42.9%）低于Ⅱ期（62.5%）和Ⅲ期（67.8%）,但差异无统计学意义（P〉0.05）。结论原发性乳腺癌外周血中可检测到CTC和TSC,CTC阳性率与临床分期和淋巴转移有相关性,TSC阳性率也随着临床分期进展阳性率提高。
Objective To observe the histopathoglocial index and prognosis of peripheral blood circulating tumor cells（ CTC） and tumor stem cells（ TSC） for patients with primary breast cancer and provide the scientific basis of clinical diagnosis and prognosis for primary breast cancer. Methods Totally 42 patients with primary cancer breast treated from January 2011 to January 2014 in our hospital were observed with peripheral blood mononuclear cell（ PBMC） while 20 healthy check-ups were selected as control group.PBMC would mark cytokeratin 19（ CK19） for peripheral blood CTC to detect the positive cells; the fluorescence microscope was applied to detect the contents of CD44＋/ CD24-/ lowCTC; the positive cells of CK19 of two groups were compared; CK19 of two groups was also compared based on clinical stages and groups. According to lymphatic metastasis,the positive cells of CK19 were observed by groups; the positive rate of CTC and TSC and correlation with clinical stages were analyzed. Results The positive rate of CK19 of observation group（ 76. 2%） was significantly higher than that of control group（ P〈0. 05）; The positive rate of N0CK19（ 59. 1%） was significantly lower than that of N1 ~ 3（ 95. 0%,P〈0. 05）; the positive rate at stage Ⅰ（ 53. 8%） was significantly lower than that of stage Ⅱ（ 84. 2%） and stage III（ 90. 0%,P〈0. 05）; the positive rate of N0CD44＋/ CD24-/ low（ 46. 2%） was lower than that of N1 ~ 3（ 78. 9%,P〈0. 05）;the positive rate at stage Ⅰ（ 42. 9%） was lower than that of stage Ⅱ（ 62. 5%） and stage Ⅲ（ 67. 8%,P〉0. 05）. Conclusions CTC and TSC from peripheral blood in primary cancer breast are detected. The positive rate of CTC is correlated with clinical staging and lymphatic metastasis. The positive rate of TSC is increased with the development of clinical stages.
出处
《中国肿瘤临床与康复》
2015年第10期1178-1180,共3页
Chinese Journal of Clinical Oncology and Rehabilitation
关键词
原发性乳腺肿瘤
外周血循环肿瘤细胞
肿瘤干细胞
组织病理学
预后
Primary breast neoplasms
Peripheral blood circulating tumor cells
Tumor stem cells
Histopathology
Prognosis
