摘要
目的观察应用兔脂肪干细胞(ADSCs)诱导的成骨细胞复合组织工程骨支架材料——猪小肠黏膜下层(SIS)在体外构建组织工程骨膜的生物学特性。方法从兔脂肪中分离ADSCs,在条件培养基下诱导分化为成骨细胞,然后将成骨细胞与SIS进行体外复合培养,检测细胞的生物学特性,并对细胞复合而成的生物活性骨膜进行碱性磷酸酶(ALP)检测。观察ADSCs与SIS复合后的生长增殖和诱导分化的情况。结果脂肪组织可获得ADSCs,贴壁后各组细胞均呈短梭形。贴壁细胞CD44阳性表达,CD29阴性表达。细胞生长曲线结果显示,细胞与SIS复合后均具有活跃的增殖能力。ALP检测显示,确定复合至组织工程骨膜上的细胞可以成骨。结论 ADSCs诱导的成骨细胞与SIS复合构建组织工程骨膜具有良好的成骨特性。
Objective To observe the biological characteristics of tissue engineered periosteum constructed by adipose- derived stem cells （ADSCs） from rabbit combined with bone tissue engineering scaffold- small intestinal submucosa （SIS） in vitro. Methods The adipose-derived stem cells were separated from rabbit adipose and induced osteoblast ad- hered on conditioned medium, osteoblast and SIS were proliferated, the active cellular function was detected. The cells composition generated biological periosteum was detected alkaline phosphatase （ALP）. The grow tendency and induction differentiation of ADSCs and combined with SIS were observed. Results ADSCs were gotten from rabbit adipose, and ADSCs after adherence showed short fusiform in vitro. The cells after adherence showed CD44 positive expression and CD29 negative expression. The results of growth curve showed osteoblast induced from ADSCs on SIS had active multi- plica tion capacity. The results of ALP detection showed osteoblast induced from ADSCs on SIS had similar osteogenic potential. Conclusion The tissue-engineered periosteum constructed by osteoblast induced from ADSCs on SIS showed good osteogenesis characteristics.
出处
《中国医药导报》
CAS
2015年第30期4-8,F0003,共6页
China Medical Herald
基金
国家自然科学基金资助项目(31170930)
关键词
脂肪干细胞
猪小肠黏膜下层
骨组织工程
Adipose-derived stem cells
Small intestinal submucosa
Bone tissue engineering
