摘要
利用线粒体控制区(Dloop区)全序列,对2013年3月~2015年4月采自中国南海6°N^20°N之间8个地理群体的200尾扁舵鲣(Auxis thazard)进行了种群遗传多样性和遗传结构分析。序列总长991 bp,包含184个多态性位点,定义了191个单倍型。遗传多样性分析表明,南海扁舵鲣总体呈现出很高的单倍型多样性(0.999 5±0.000 6)和较高的核苷酸多样性(0.019±0.009)。系统发育分析、分子方差分析和成对遗传分化系数(FST)分析显示,南海范围内扁舵鲣不存在与地理群体对应的支系,群体间存在很强的基因流,遗传分化不显著。种群动态的中性检验和核苷酸不配对分布分析表明,南海扁舵鲣历史上曾经历过种群的快速扩张。结果表明,南海扁舵鲣遗传多样性丰富,具备较高的生态适应和进化能力;南海扁舵鲣属于同一个随机交配的种群,在渔业开发和管理上可视为一个单一的管理单元。
We examined the population genetic structure and genetic diversity of the frigate tuna（ Auxis thazard） in the South China Sea based on mitochondrial control region（ Dloop） sequences,using a total of 200 individuals collected from eight sites（ 6°N - 20°N）during March 2013 and April 2015. Fragments of 911 bp were sequenced and a total of 184 polymorphic sites were detected,which defined 191 haplotypes. Genetic diversity analysis shows that A. thazard in the South China Sea were characterized by rather high haplotype diversity（ 0. 999 5 ± 0. 000 6） and high nucleotide diversity（ 0. 019 ± 0. 009）. Phylogenetic analysis reveals no significant genealogical clades of samples corresponding to sampling localities. Analyses of molecular variance and pairwise FSTsuggest a high rate of gene flow and the lack of genetic differentiation among different populations. Both neutrality tests and mismatch distribution analyses indicate a recent population expansion in A. thazard. It is demonstrated that A. thazard in the South China Sea belong to the same population,with strong adaptive capacity and evolutionary potential due to its high genetic diversity. Thus a single-stock management regime could be supported in fishery exploitation and management.
出处
《南方水产科学》
CAS
CSCD
北大核心
2015年第5期82-89,共8页
South China Fisheries Science
基金
农业部财政重大专项(NFZX2013)
国家重点基础研究发展计划(973计划)项目(2014CB441500)
国家科技支撑计划项目(2013BAD13B06)
中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院南海水产研究所)资助项目(2014TS17)
关键词
扁舵鲣
种群遗传结构
遗传多样性
种群管理
南海
Auxis thazard
population genetic structure
genetic diversity
population management
the South China Sea
