摘要
目的研究中药对体外培养的人瘢痕组织皮肤成纤维细胞生长的影响,筛选出抗瘢痕有效的中药及其作用浓度。方法用四甲基偶氮唑盐比色试验和生长曲线测定法,比较单味中药醇提物对肥厚性瘢痕成纤维细胞(Fb)和人正常皮肤Fb的体外生长活力和生长状态的影响。结果①五倍子可明显抑制Fb的体外增殖率,并且对肥厚性瘢痕Fb的作用较正常皮肤Fb强。②五倍子生药醇提取物浓度在0～400μg/mL时,对肥厚性瘢痕Fb增殖率的抑制作用与药物剂量呈明显的正相关,其对肥厚性瘢痕Fb的半数抑制浓度为100μg/mL(生药);在<400μg/mL浓度时,与秋水仙碱比较,细胞形态无明显改变。③50μg/mL的五倍子可延迟并缩短正常皮肤Fb和肥厚性瘢痕Fb的对数生长期,延长Fb倍增时间。对肥厚性瘢痕Fb和正常皮肤Fb的生长抑制率与药物的作用时间相依赖。结论中药五倍子对肥厚性瘢痕Fb体外生长增殖的抑制作用明显地与时间和剂量相依赖,为外用该药治疗瘢痕提供了实验依据。
Objective To study the effect of Chinese traditional medicines on human hypertrophic scar fibroblasts in vitro,and determine their effective dosage.Methods Traditional Chinese medicines were cultured with human hypertrophic scar fibrob lasts(Fb)and normal dermal Fb,MTT colorimetr ic assay and cell growth curve were used to compare the influence of ethanol extracts fromChinese tradi tional medicines on the Fb growth activity and growth cycle.Results(1)Chinese gall markedly inhibited the proliferation of Fb derived from hyp ertrophic scar and normal skin,and was more effective on the former in a dose-dependent m anner(0～400μg /mL)during 72h of treatment.(2)The half inhibition concentration o f Chinese gall ethanol extract was 100μg /mL.The morphology of Fb did not change when its concentration was below 400μg /mL,as compared to colchicine(<50μg /mL).(3)Chinese gall at 50μg /mL concentration delayed and shortened the Log phase and extended the p opulation doubling time (PDT)in cultured normal human and hypertr ophic scar skin Fb in a manner of time-dependent.Conclusions Chinese gall can inhibit the prolife ration of human fibroblasts derived fromhypertrophic scar and normal hu man skin.The dose-and time-dependent inhibi tion suggest that Chinese gall could be investigated as a novel potential thera-peutic agent in treating abnormal dermal scarring.[
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2002年第4期299-301,共3页
Chinese Journal of Dermatology
基金
江苏省社会发展基金资助(BS2000398)
关键词
肥大性瘢痕
成纤维细胞
红花
五倍子
蜈蚣
瘢痕疙瘩
药理学
Cicatrix,hypertrophic
Fibroblasts
Carthamus tinctorius
Galla chi nensis
Scolopendra subspinipes
