摘要
目的:探讨BIOMED-2系统在检测B细胞非霍奇金淋巴瘤中的应用价值。方法:选取石蜡包埋组织B细胞非霍奇金淋巴瘤40例、其他淋巴结增生性病变10例,提取组织DNA,应用BIOMED-2系统中的61条引物分成9个组别进行PCR扩增,检测DNA质量及抗原受体基因重排克隆性。结果:94%(47/50)样本DNA长度在300 bp以上,BIOMED-2系统检测的敏感性和特异性均为100%,Ig H-A、Ig H-B、Ig H-C、Ig H-D、Ig H-E、Ig K-A、Ig K-B、Ig L组检出率分别为45%(18/40)、27.5%(11/40)、87.5%(35/40)、55%(22/40)、47.5%(19/40)、80%(32/40)、50%(20/40)、22.5%(9/40),Ig H-C联合Ig K-A组合检出率为100%。结论:BIOMED-2系统适用于石蜡包埋组织B细胞抗原受体基因重排检测,具有很高的敏感性和特异性。
Objective： To evaluate the BIOMED-2 system in detecting the monoclonality of antigen receptor gene rearrangement in B-cell Non-Hodgkin lymphoma. Methods： Genomic DNA was extracted from paraffin embedded specimens of 40 cases of B cell Non-Hodgkin lymphoma and 10 cases of proliferative diseases of lymph nodes, and clonality of antigen receptor gene rearrangement was assessed using the 9 groups of total 61 primers of BIOMED-2 system. Results： 94% （47/50） of specimens yielded PCR products over 300 bp using DNA quality control primers. The sensitivity and specificity of BIOMED-2 system in the detection of monoclona- lity of antigen receptor gene rearrangement were both 100%. The rate of detection of monoclonality using primes for IgH-A, IgH 7B, IgH-C, IgH-D, IgH-E, IgK-A, IgK-B, IgL was 45% （18/40）, 27.5 % （11/40）, 87.5% （35/40）, 55 % （22/40）, 47. 5 % （ 19/40 ）, 80% （ 32/40 ）, 50% （ 20/40 ） and 22.5 % （9/40） respectively. When combining IgH -C and IgK-A primers, all cases of antigen receptor gene monoclonality rearrangement could be detected. Conclusion： BIOMED-2 system is a highly sensitive and specific method in detecting the monoclonality of antigen receptor gene rearrangement of B cell Non-Hodgkin lymphoma in routine paraffin embedded specimens.
出处
《泸州医学院学报》
2015年第5期437-440,共4页
Journal of Luzhou Medical College
