摘要
本试验旨在研究长链非编码RNA（H19）在骨骼肌发育和损伤修复过程中表达量的变化,为研究H19在小鼠骨骼肌发育中的作用机制奠定基础。以C2C12细胞系和ICR小鼠为试验素材,通过生物信息学方法分析H19的非编码特性和在物种间的低保守性,通过实时荧光定量PCR（qRT-PCR）方法检测H19在C2C12细胞分化,ICR小鼠骨骼肌发育不同时期和成体小鼠骨骼肌损伤修复过程中表达量的动态变化。结果表明,H19在小鼠骨骼肌中的表达量随出生后日龄的增长逐渐下降;在诱导C2C12细胞分化过程中,H19的表达量先是逐渐升高,进而在较高水平维持不变;在肌肉损伤模型中修复的第4~6天,H19的表达量维持在较高水平。鉴于上述H19在C2C12细胞分化和肌损伤修复过程中的表达特点,推断H19可能具有促进肌纤维形成和成体骨骼肌损伤后修复的功能。
This experiment was intend to study the changes of long non-coding RNA（H19）expression levels in skeletal muscle development and regeneration,and lay the foundation of its mechanism reach in skeletal muscle development.C2C12 cell line and ICR mice were used as experimental material,bioinformatics assay was used to exploit its non-coding character and low conservatism in different species,and the expressions of H19 in C2C12cell differentiation,skeletal muscle development and the phase of muscle regeneration were detected by quantitative Real-time PCR（qRT-PCR）.The results showed that,H19 expression levels in postnatal mouse skeletal muscle decreased with increasing age;during C2C12 cell differentiation,H19 mRNA increased gradually,and then maintained a high level;The expression of H19 was maintained at a high level through days 4to 6after injury.In consideration of its express character in C2C12 cell differentiation and skeletal muscle damage repair model,H19 may play an important role in promoting myogenesis and skeletal muscle regeneration.
出处
《中国畜牧兽医》
CAS
北大核心
2015年第10期2521-2528,共8页
China Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金(31372295)
国家"973"(2012CB124706)
中国农业科学院科技创新工程(ASTIP-IAS05)
